蓖麻GATA基因家族的鉴定和特征分析

doi:10.7677/ynzwyj201514151

摘要/Abstract

摘要：

GATA因子在植物中广泛参与了光响应调控、叶绿素合成以及碳、氮代谢等与作物产量息息相关的生物学过程。本研究基于蓖麻基因组信息，鉴定了19个蓖麻GATA家族成员，根据基因结构、蛋白结构和系统进化分析将其分为4个亚家族。理化性质分析发现GATA蛋白具有亲水性。基因和蛋白结构分析发现，蓖麻与拟南芥的GATA成员在结构上非常保守。利用高通量RNAseq测序数据分析蓖麻GATA基因在各个组织中的表达情况，发现GATA基因主要在根和叶中表达，在种子中表达较低。进一步通过半定量RTPCR技术检测GATA基因在黑暗下的表达情况，结果显示有三个GATA基因在黑暗处理后表达下调，预示着蓖麻GATA基因参与了光响应调控过程。这些信息为蓖麻GATA基因家族的功能分析和提高蓖麻产量提供了理论基础。

关键词: 蓖麻, GATA基因家族, 基因表达, 黑暗处理

Abstract:

GATA proteins are considered to be broadly involved in yieldassociated biological process, such as photoresponse, chlorophyll biosynthesis, and carbon and nitrogen metabolism. Based on castor bean genome database, a total of 19 GATA genes were identified and classified into 4 subfamilies according to gene structure, protein structure and their phylogenetic relationships. Results exhibited that GATA factors were hydrophilic proteins. Analysis of gene structure and protein structure revealed the conserved structural features of GATA factors between castor bean and Arabidopsis thaliana. The highthroughput RNAseq data were used to define the expressional profiles of GATA genes among tissues. The results showed that most of the castor GATA genes preferentially expressed in leaf and root in contrast to their expression in developing seeds. In particular, the expression of GATA genes responding to darkness treatment in leaves was detected using semiquantitative RTPCR. It was shown that expression of three genes was downregulated under darkness treatment, which suggests a role for GATA genes of castor bean in lightmediated regulation. These results provide important theoretical basis to the functions identification of castor GATA genes and increase castor yields.

Key words: Castor bean, GATA gene family, Gene expression, Dark treatment

中图分类号:

Q 75

敖涛1、3, 廖晓佳2、3, 徐伟2、3, 刘爱忠1. 蓖麻GATA基因家族的鉴定和特征分析[J]. Plant Diversity, 2015, 37(4): 453-462.

AO Tao-, LIAO Xiao-Jia-, XU Wei-, LIU Ai-Zhong. Identification and Characterization of GATA Gene Family in Castor Bean (Ricinus communis)[J]. Plant Diversity, 2015, 37(4): 453-462.

参考文献

黄文霞, 何觉民, 陆建农, 2006. 国内外蓖麻杂种优势利用和蓖麻加工研究现状［J］. 作物研究, (5): 541—544
吕凤霞, 刘恩魁, 刘永平, 1994. 蓖麻的开发利用前途广阔［J］. 河北农业科技, 4 (2): 12
An Y, Han X, Tang S et al., 2014. Poplar GATA transcription factor PdGNC is capable of regulating chloroplast ultrastructure, photosynthesis, and vegetative growth in Arabidopsis under varying nitrogen levels［J］. Plant Cell, Tissue and Organ Culture
Aravind L, Iyer LM, 2012. The HAREHTH and associated domains［J］. Cell Cycle, 11 (1): 119—131
Bai Y, Meng Y, Huang D et al., 2011. Origin and evolutionary analysis of the plantspecific TIFY transcription factor family［J］. Genomics, 98 (2): 128—136
Bi YM, Zhang Y, Signorelli T et al., 2005. Genetic analysis of Arabidopsis GATA transcription factor gene family reveals a nitrateinducible member important for chlorophyll synthesis and glucose sensitivity［J］. The Plant Journal, 44 (4): 680—692
Borello U, Ceccarelli E, Giuliano G, 1993. Constitutive, lightresponsive and circadian clockresponsive factors compete for the different l box elements in plant lightregulated promoters［J］. The Plant Journal, 4 (4): 611—619
Chiang YH, Zubo YO, Tapken W et al., 2012. Functional Characterization of the GATA Transcription Factors GNC and CGA1 Reveals Their Key Role in Chloroplast Development, Growth, and Division in Arabidopsis［J］. Plant Physiology, 160 (1): 332—348
Chini A, Fonseca S, Chico JM et al., 2009. The ZIM domain mediates homo and heteromeric interactions between Arabidopsis JAZ proteins［J］. The Plant Journal, 59 (1): 77—87
DanielVedele F,Caboche M,1993.A tobacco cDNA clone encoding a GATA1 zinc finger protein homologous to regulators of nitrogen metabolism in fungi［J］.Molecular and General Genetics,240(3):365—373
GelsiBoyer V, Brecqueville M, Devillier R et al., 2012. Mutations in ASXL1 are associated with poor prognosis across the spectrum of malignant myeloid diseases［J］. Journal of Hematology & Oncology, 5: 12
Griffiths S, Dunford RP, Coupland G et al., 2003. The evolution of CONSTANSlike gene families in barley, rice, and Arabidopsis［J］. Plant Physiology, 131 (4): 1855—1867
Hudson D, Guevara DR, Hand AJ et al., 2013. Rice cytokinin GATA transcription Factor1 regulates chloroplast development and plant architecture［J］. Plant Physiology, 162 (1): 132—144
Hudson D, Guevara DR, Yaish MW et al., 2011. GNC and CGA1 modulate chlorophyll biosynthesis and glutamate synthase (GLU1/FdGOGAT) expression in Arabidopsis［J］. PLoS ONE, 6 (11):1—16
Hudson ME, Lisch DR, Quail PH, 2003. The FHY3 and FAR1 genes encode transposaserelated proteins involved in regulation of gene expression by the phytochrome Asignaling pathway［J］. The Plant Journal, 34 (4): 453—471
Jeong MJ,Shih MC,2003.Interaction of a GATA factor with cisacting elements involved in light regulation of nuclear genes encoding chloroplast glyceraldehyde3phosphate dehydrogenase in Arabidopsis［J］. Biochemical and Biophysical Research Communication, 300 (2): 555—562
Jin ZW, Xu W, Liu AZ, 2013. Genomic surveys and expression analysis of bZIP gene family in castor bean (Ricinus communis L.) ［J］. Planta, 239 (2): 299—312
Katoh M, 2013. Functional and cancer genomics of ASXL family members［J］. British Journal of Cancer, 109 (2): 299—306
Kiba T, Naitou T, Koizumi N et al., 2005. Combinatorial microarray analysis revealing Arabidopsis genes implicated in cytokinin Responses through the His→Asp Phosphorelay Circuitry［J］. Plant Cell Physiology, 46 (2): 339—355
Lisch DR, Freeling M, Langham RJ et al., 2001. Mutator transposase is widespread in the grasses［J］. Plant Physiology, 125 (3): 1293—1303
Liu PP, Koizuka N, Martin RC et al., 2005. The BME3 (Blue Micropylar End 3) GATA zinc finger transcription factor is a positive regulator of Arabidopsis seed germination［J］. The Plant Journal, 44 (6): 960—971
Lowry JA, Atchley WR, 2000. Molecular evolution of the GATA family of transcription factors: conservation within the DNAbinding Domain［J］. Journal of Molecular Evolution, 50 (2): 103—115
Luo XM, Lin WH, Zhu SW et al., 2010. Integration of Light and BrassinosteroidSignaling Pathways by a GATA Transcription Factor in Arabidopsis［J］. Developmental Cell, 19 (6): 872—883
Manfield LW, Devlin PF, Jen CH et al., 2007. Conservation, convergence, and divergence of lightresponsive, circadianregulated, and tissuespecific expression patterns during evolution of the Arabidopsis GATA gene family［J］. Plant Physiology, 143 (2): 941—958
Mara CD, Irish VF, 2008. Two GATA transcription factors are downstream effectors of floral homeotic gene action in Arabidopsis［J］. The Plant Physiology, 147 (2):707—718
Melotto M, Mecey C, Niu Y et al., 2008. A critical role of two positively charged amino acids in the Jas motif of Arabidopsis JAZ proteins in mediating coronatine and jasmonoyl isoleucinedependent interactions with the COI1 Fbox protein［J］. The Plant Journal, 55 (6): 979—988
Naito T, Kiba T, Koizumi N et al., 2007. Characterization of a unique GATA family gene that responds to both light and cytokinin in Arabidopsis thaliana［J］. Bioscience, Biotechnology, and Biochemistry, 71 (6): 1557—1560
Omichinski JG, Clore GM, Schaad O et al., 1993. NMR structure of a specific DNA complex of Zncontaining DNA binding domain of GATA1［J］. Science, 261 (5120): 438—446
Patient RK, McGhee JD, 2002. The GATA family (vertebrates and invertebrates) ［J］. Curr Opin Genet Dev, 12 (4): 416—422
Reyes JC, MuroPastor MI, Florencio FJ, 2004. The GATA family of transcription factors in Arabidopsis and rice［J］. Plant Physiology, 134 (4): 1718—1732
Robson F, Costa MM, Hepworth SR et al., 2001. Functional importance of conserved domains in the floweringtime gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants［J］. The Plant Journal, 28 (6): 619—631
Scazzocchio C, 2000. The fungal GATA factors［J］. Current Opinion in Microbiology, 3 (2):126—131
Scheible WR, Morcuende R, Czechowski T et al., 2004. Genomewide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen［J］. Plant Physiology, 136 (1): 2483—2499
Schindler U, Cashmore AR, 1990. Photoregulated gene expression may involve ubiquitous DNA binding proteins［J］. EMBO Journal, 9 (11): 3415—3427
Starich MR, Wikstrom M, Arst HN Jr et al., 1998. The solution structure of a fungal AREA proteinDNA complex: an alternative binding mode for the basic carboxyl tail of GATA factors［J］. Journal of Molecular Biology, 277 (3): 605—620
Teakle GR, Gilmartin PM, 1998. Two forms of type IV zincfinger motif and their kingdomspecific distribution between the flora, fauna and fungi［J］. Trends in Biochemical Sciences, 23 (3): 100—102
Teakle GR, Manfield IW, Graham JF et al., 2002. Arabidopsis thaliana GATA factors: organization, expression and DNAbinding characteristics［J］. Plant Molecular Biology, 50 (1): 43—57
Terzaghi WB, Cashmore AR, 1995. Lightregulated transcription［J］. Annual Review of Plant Physiology and Plant Molecular Biology, 46: 445—474
Vanholme B, Grunewald W, Bateman A et al., 2007. The tify family previously known as ZIM［J］. Trends in Plant Science, 12 (6): 239—244
Wang R, Okamoto M, Xing X et al., 2003. Microarray analysis of the nitrate response in Arabidopsis roots and shoots reveals over 1,000 rapidly responding genes and new linkages to glucose, trehalose6phosphate, iron, and sulfate metabolism［J］. Plant Physiology, 132 (2): 556—567
Xu W, Li F, Ling LZ et al., 2013. Genomewide survey and expression profiles of the AP2/ERF family in castor bean (Ricinus communis L.) ［J］. BMC Genomics, 14 (13): 785
Zhu BG (朱保葛), Li WB (李文彬), 2007. Progress in castor biotechnology［J］. Chinese Biotechnology (中国生物工程杂志), 27 (10): 98—102

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