Seed dormancy and germination characteristics of two Rheum species in the Himalaya-Hengduan Mountains

doi:10.1016/j.pld.2017.05.009

Plant Diversity ›› 2017, Vol. 39 ›› Issue (04): 180-186.DOI: 10.1016/j.pld.2017.05.009

Seed dormancy and germination characteristics of two Rheum species in the Himalaya-Hengduan Mountains

Deli Peng^a,b, Zhe Chen^a,d, Xiaojian Hu^c, Zhimin Li^b, Bo Song^a, Hang Sun^a

a Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;
b School of Life Science, Yunnan Normal University, Kunming 650500, Yunnan, China;
c Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;
d University of Chinese Academy of Sciences, Beijing 100049, China

收稿日期:2017-03-04 修回日期:2017-05-27 出版日期:2017-08-25 发布日期:2021-11-05
通讯作者: Bo Song, Hang Sun
基金资助:
This study was supported by National Key Research and Development Program of China (2017YFC0505201), NSFC (grant 31670206 to Z.-M. L. and 31570228 to B. S.), major Program of NSFC (grant 31590823 to H. S.), the Orientation Training Programme for Postdoctoral Fellows from Yunnan Province to D.-L. P., and the Young Academic and Technical Leader Raising Foundation of Yunnan Province to B. S.

Seed dormancy and germination characteristics of two Rheum species in the Himalaya-Hengduan Mountains

Deli Peng^a,b, Zhe Chen^a,d, Xiaojian Hu^c, Zhimin Li^b, Bo Song^a, Hang Sun^a

a Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;
b School of Life Science, Yunnan Normal University, Kunming 650500, Yunnan, China;
c Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;
d University of Chinese Academy of Sciences, Beijing 100049, China

Received:2017-03-04 Revised:2017-05-27 Online:2017-08-25 Published:2021-11-05
Contact: Bo Song, Hang Sun
Supported by:
This study was supported by National Key Research and Development Program of China (2017YFC0505201), NSFC (grant 31670206 to Z.-M. L. and 31570228 to B. S.), major Program of NSFC (grant 31590823 to H. S.), the Orientation Training Programme for Postdoctoral Fellows from Yunnan Province to D.-L. P., and the Young Academic and Technical Leader Raising Foundation of Yunnan Province to B. S.

摘要/Abstract

摘要： Seed dormancy and germination characteristics are important factors determining plant reproductive success, and may be expected to have a major influence on plant distribution. In this study, we aimed to explore the characteristics of seed dormancy and germination in two endemic Rheum species (Rheum nobile and Rheum alexandrae) in the Himalaya-Hengduan Mountains. To determine the type of dormancy, fresh seeds of the two species (one population each) were incubated in light at 25/15 and 15/5℃, and then dry after-ripening (DAR) seeds were incubated on water agar substrate with or without GA3. To determine the effect of temperature and light on germination, DAR seeds of the two species (two populations each) were incubated both in the light and in the dark at several temperatures, including constant and alternating temperatures. Base temperature (T_b) and thermal times for 50% germination (θ₅₀) were calculated. DAR released physiological dormancy (PD), increasing final germination at 15/5℃ and widening the range of germination temperatures from higher to lower, indicative of type 2 non-deep PD for the two Rheum species. Light had no significant effect on germination of seeds from the two species (two populations each). Seeds of the two species germinated significantly better (>80%) at medium temperatures (10-25℃) than at extreme low (5℃) or high (35℃) temperatures. Alternating temperatures (25/15 and 15/5℃) did not significantly increase the final germination of the two species either in the light and in the dark, but it promoted seed germination more quickly than corresponding constant temperatures in the light in both Rh. alexandrae populations, especially at 15/5℃. Germination in response to temperature was well described by the thermal-time model at suboptimal temperatures. The estimated T_b values were 1 and 0.9℃, respectively, in two Rh. nobile populations; 4 and 4.1℃, respectively, in two Rh. alexandrae populations; θ₅₀ (thermal time) were 100 and 125 Cd, respectively in two Rh. nobile populations; 76.92 and 83.33 Cd, respectively in two Rh. alexandrae populations. The dormancy type, and germination responses to temperature and light condition does not explain why the two Rheum species are distributed in contrasting habitats. However, these findings reflect an advantageous germination strategy of these two Rheum species to adapt to the same alpine environments.

关键词: Germination, Light, Rheum alexandrae, Rheum nobile, Dormancy, Temperature

Abstract: Seed dormancy and germination characteristics are important factors determining plant reproductive success, and may be expected to have a major influence on plant distribution. In this study, we aimed to explore the characteristics of seed dormancy and germination in two endemic Rheum species (Rheum nobile and Rheum alexandrae) in the Himalaya-Hengduan Mountains. To determine the type of dormancy, fresh seeds of the two species (one population each) were incubated in light at 25/15 and 15/5℃, and then dry after-ripening (DAR) seeds were incubated on water agar substrate with or without GA3. To determine the effect of temperature and light on germination, DAR seeds of the two species (two populations each) were incubated both in the light and in the dark at several temperatures, including constant and alternating temperatures. Base temperature (T_b) and thermal times for 50% germination (θ₅₀) were calculated. DAR released physiological dormancy (PD), increasing final germination at 15/5℃ and widening the range of germination temperatures from higher to lower, indicative of type 2 non-deep PD for the two Rheum species. Light had no significant effect on germination of seeds from the two species (two populations each). Seeds of the two species germinated significantly better (>80%) at medium temperatures (10-25℃) than at extreme low (5℃) or high (35℃) temperatures. Alternating temperatures (25/15 and 15/5℃) did not significantly increase the final germination of the two species either in the light and in the dark, but it promoted seed germination more quickly than corresponding constant temperatures in the light in both Rh. alexandrae populations, especially at 15/5℃. Germination in response to temperature was well described by the thermal-time model at suboptimal temperatures. The estimated T_b values were 1 and 0.9℃, respectively, in two Rh. nobile populations; 4 and 4.1℃, respectively, in two Rh. alexandrae populations; θ₅₀ (thermal time) were 100 and 125 Cd, respectively in two Rh. nobile populations; 76.92 and 83.33 Cd, respectively in two Rh. alexandrae populations. The dormancy type, and germination responses to temperature and light condition does not explain why the two Rheum species are distributed in contrasting habitats. However, these findings reflect an advantageous germination strategy of these two Rheum species to adapt to the same alpine environments.

Key words: Germination, Light, Rheum alexandrae, Rheum nobile, Dormancy, Temperature

Deli Peng, Zhe Chen, Xiaojian Hu, Zhimin Li, Bo Song, Hang Sun. Seed dormancy and germination characteristics of two Rheum species in the Himalaya-Hengduan Mountains[J]. Plant Diversity, 2017, 39(04): 180-186.

参考文献

Baskin, C.C., Baskin, J.M., 2014. Seeds:Ecology, Biogeography, and Evolution of Dormancy and Germination. Elsevier Academic Press Inc, San Diego.
Baskin, J.M., Baskin, C.C., 2004. A classification system for seed dormancy. Seed Sci.Res. 14, 1-16.
Batlla, D., Benech-Arnold, R.L., 2015. A framework for the interpretation of temperature effects on dormancy and germination in seed populations showing dormancy. Seed Sci. Res. 25, 147-158.
Brown, R.F., Mayer, D.G., 1988. Representing cumulative germination. 2. The use of the Weibull function and other empirically derived curves. Ann. Bot. 61,127-138.
Chowdhery, H., Agrawala, D., 2009. Rheum nobile Hook. f. & Thoms. (Polygonaceae) -a rare and highly specialized plant of Himalayan region. Indian J. For. 32,145-148.
Dürr, C., Dickie, J.B., Yang, X.Y., et al., 2015. Ranges of critical temperature and water potential values for the germination of species worldwide:contribution to a seed trait database. Agric. For. Meteorol. 200, 222-232.
Densmore, R.V., 1997. Effect of day length on germination of seeds collected in Alaska. Am. J. Bot. 84, 274-278.
Garciahuidobro, J., Monteith, J.L., Squire, G.R., 1982. Time, temperature and germination of Pearl Millet (Pennisetum typhoides S. & H.) I. Constant temperature.J. Exp. Bot. 33, 288-296.
Grime, J.P., Mason, G., Curtis, A.V., et al., 1981. A comparative-study of germination characteristics in a local flora. J. Ecol. 69, 1017-1059.
Hardegree, S.P., 2006. Predicting germination response to temperature. I. Cardinaltemperature models and subpopulation-specific regression. Ann. Bot. 97,1115-1125.
Hu, X.W., Fan, Y., Baskin, C.C., et al., 2015. Comparison of the effects of temperature and water potential on seed germination of Fabaceae species from desert and subalpine grassland. Am. J. Bot. 102, 649-660.
Jaganathan, G.K., Dalrymple, S.E., Liu, B., 2015. Towards an understanding of factors controlling seed bank composition and longevity in the alpine environment. Bot. Rev. 81, 70-103.
Kudo, G., Kimura, M., Kasagi, T., et al., 2010. Habitat-specific responses of alpine plants to climatic amelioration:comparison of fellfield to snowbed communities. Arct. Antarct. Alp. Res. 42, 438-448.
Liu, K., Baskin, J.M., Baskin, C.C., et al., 2013. Effect of diurnal fluctuating versus constant temperatures on germination of 445 species from the eastern Tibet Plateau. PLoS One 8, e69364.
Omori, Y., Ohba, H., 1996. Pollen development of Rheum nobile Hook f & Thomson (Polygonaceae), with reference to its sterility induced by bract removal. Bot. J. Linn. Soc. 122, 269-278.
Peng, D.L., Hu, X.J., Yang, J., et al., 2017. Seed dormancy, germination and soil seed bank of Lamiophlomis rotata and Marmoritis complanatum (Labiatae), two endemic species from HimalayaeHengduan Mountains. Plant Biosyst. http://dx.doi.org/10.1080/11263504.2017.1311959.
Schwienbacher, E., Navarro-Cano, J.A., Neuner, G., et al., 2011. Seed dormancy in alpine species. Flora 206, 845-856.
Song, B., Stocklin, J., Gao, Y.Q., et al., 2013a. Habitat-specific responses of seed germination and seedling establishment to soil water condition in two Rheum species in the high Sino-Himalayas. Ecol. Res. 28, 643-651.
Song, B., Zhang, Z.Q., Stocklin, J., et al., 2013b. Multifunctional bracts enhance plant fitness during flowering and seed development in Rheum nobile (Polygonaceae),a giant herb endemic to the high Himalayas. Oecologia 172, 359-370.
Song, B., Stoecklin, J., Peng, D.L., et al., 2015. The bracts of the alpine 'glasshouse' plant Rheum alexandrae (Polygonaceae) enhance reproductive fitness of its pollinating seed-consuming mutualist. Bot. J. Linn. Soc. 179, 349-359.
Sun, Y.S., Wang, A.L., Wan, D.S., et al., 2012. Rapid radiation of Rheum (Polygonaceae) and parallel evolution of morphological traits. Mol. Phylogenet. Evol. 63,150-158.
Thompson, K., Grime, J.P., 1983. A comparative study of germination responses to diurnally fluctuating temperatures. J. Appl. Ecol. 20, 141-156.
Tsukaya, H., 2002. Optical and anatomical characteristics of bracts from the Chinese "glasshouse" plant, Rheum alexandrae Batalin (Polygonaceae), in Yunnan, China. J. Plant Res. 115, 59-63.
Xu, B., Li, Z.M., Sun, H., 2014. Seed Plants of the Alpine Subnival Belt from the Hengduan Mountains, SW China. Science Press, Beijing.

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Seed dormancy and germination characteristics of two Rheum species in the Himalaya-Hengduan Mountains

Seed dormancy and germination characteristics of two Rheum species in the Himalaya-Hengduan Mountains

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