Development and characterization of 43 microsatellite markers for the critically endangered primrose Primula reinii using MiSeq sequencing

doi:10.1016/j.pld.2017.09.003

Plant Diversity ›› 2018, Vol. 40 ›› Issue (01): 41-44.DOI: 10.1016/j.pld.2017.09.003

• Articles • 上一篇

Development and characterization of 43 microsatellite markers for the critically endangered primrose Primula reinii using MiSeq sequencing

Masaya Yamamoto^a, Yoshihiro Handa^,b, Hiroki Aihara^b, Hiroaki Setoguchi^a

a Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu, Sakyo-ku, Kyoto 606-8501, Japan;
b FASMAC Co., Ltd., 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan

收稿日期:2017-03-23 修回日期:2017-09-06 出版日期:2018-02-25 发布日期:2021-11-05
通讯作者: Masaya Yamamoto
基金资助:
This research was financially and technically supported by FASMAC Co., Ltd. (Kanagawa, Japan) and The Environment Research and Technology Development Fund (#4-1403).

Development and characterization of 43 microsatellite markers for the critically endangered primrose Primula reinii using MiSeq sequencing

Masaya Yamamoto^a, Yoshihiro Handa^,b, Hiroki Aihara^b, Hiroaki Setoguchi^a

a Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu, Sakyo-ku, Kyoto 606-8501, Japan;
b FASMAC Co., Ltd., 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan

Received:2017-03-23 Revised:2017-09-06 Online:2018-02-25 Published:2021-11-05
Contact: Masaya Yamamoto
Supported by:
This research was financially and technically supported by FASMAC Co., Ltd. (Kanagawa, Japan) and The Environment Research and Technology Development Fund (#4-1403).

摘要/Abstract

摘要： Primula reinii (Primulaceae), a perennial herb belonging to the Primula section Reinii, occurs on wet, shaded rocky cliffs in the mountains of Japan. This threatened species comprises four varieties; these plants are very localized and rare in the wild. In this study, 43 microsatellite markers were developed using MiSeq sequencing to facilitate conservation genetics of these critically endangered primroses. We developed novel microsatellite markers for three varieties of P. reinii, and tested its polymorphism and genetic diversity using natural populations. These novel markers displayed relatively high polymorphism; the number of alleles and expected heterozygosities ranged from 2 to 6 (mean=3.2) and 0.13 to 0.82 (mean=0.45), respectively. All loci were in HardyeWeinberg equilibrium. These microsatellite markers will be powerful tools to assess P. reinii genetic diversity and develop effective conservation and management strategies.

关键词: Microsatellites, Polymorphism, MiSeq, Critically endangered plant, Primula reinii

Abstract: Primula reinii (Primulaceae), a perennial herb belonging to the Primula section Reinii, occurs on wet, shaded rocky cliffs in the mountains of Japan. This threatened species comprises four varieties; these plants are very localized and rare in the wild. In this study, 43 microsatellite markers were developed using MiSeq sequencing to facilitate conservation genetics of these critically endangered primroses. We developed novel microsatellite markers for three varieties of P. reinii, and tested its polymorphism and genetic diversity using natural populations. These novel markers displayed relatively high polymorphism; the number of alleles and expected heterozygosities ranged from 2 to 6 (mean=3.2) and 0.13 to 0.82 (mean=0.45), respectively. All loci were in HardyeWeinberg equilibrium. These microsatellite markers will be powerful tools to assess P. reinii genetic diversity and develop effective conservation and management strategies.

Key words: Microsatellites, Polymorphism, MiSeq, Critically endangered plant, Primula reinii

Masaya Yamamoto, Yoshihiro Handa, Hiroki Aihara, Hiroaki Setoguchi. Development and characterization of 43 microsatellite markers for the critically endangered primrose Primula reinii using MiSeq sequencing[J]. Plant Diversity, 2018, 40(01): 41-44.

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Development and characterization of 43 microsatellite markers for the critically endangered primrose Primula reinii using MiSeq sequencing

Development and characterization of 43 microsatellite markers for the critically endangered primrose Primula reinii using MiSeq sequencing

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