The molecular mechanism of WRINKLED1 transcription factor regulating oil accumulation in developing seeds of castor bean

doi:10.1016/j.pld.2022.09.003

Plant Diversity ›› 2023, Vol. 45 ›› Issue (04): 469-478.DOI: 10.1016/j.pld.2022.09.003

The molecular mechanism of WRINKLED1 transcription factor regulating oil accumulation in developing seeds of castor bean

Qing Tan^a,e, Bing Han^b, Mohammad Enamul Haque^a, Ye-Lan Li^d, Yue Wang^a, Di Wu^b, Shi-Bo Wu^a, Ai-Zhong Liu^c

a. Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China;
b. Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;
c. Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China (Ministry of Education), Southwest Forestry University, Kunming 650224, China;
d. College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China;
e. University of Chinese Academy of Sciences, Beijing 100101, China

收稿日期:2022-07-11 修回日期:2022-08-23 出版日期:2023-07-25 发布日期:2023-08-21
通讯作者: Ai-Zhong Liu,E-mail:liuaizhong@mail.kib.ac.cn
基金资助:
This work was supported by National Natural Science Foundation of China (grant number 31701465). We are grateful to the Service Center for Experimental Biotechnology at the Kunming Institute of Botany, CAS, for supporting plant cultivation.

The molecular mechanism of WRINKLED1 transcription factor regulating oil accumulation in developing seeds of castor bean

Qing Tan^a,e, Bing Han^b, Mohammad Enamul Haque^a, Ye-Lan Li^d, Yue Wang^a, Di Wu^b, Shi-Bo Wu^a, Ai-Zhong Liu^c

a. Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China;
b. Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;
c. Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China (Ministry of Education), Southwest Forestry University, Kunming 650224, China;
d. College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China;
e. University of Chinese Academy of Sciences, Beijing 100101, China

Received:2022-07-11 Revised:2022-08-23 Online:2023-07-25 Published:2023-08-21
Contact: Ai-Zhong Liu,E-mail:liuaizhong@mail.kib.ac.cn
Supported by:
This work was supported by National Natural Science Foundation of China (grant number 31701465). We are grateful to the Service Center for Experimental Biotechnology at the Kunming Institute of Botany, CAS, for supporting plant cultivation.

摘要/Abstract

摘要： The transcription factor WRINKLED1 (WRI1), a member of AP2 gene family that contain typical AP2 domains, has been considered as a master regulator regulating oil biosynthesis in oilseeds. However, the regulatory mechanism of RcWRI1 in regulating oil accumulation during seed development has not been clearly addressed. Castor bean (Ricinus communis) is one of the most important non-edible oil crops and its seed oils are rich in hydroxy fatty acids, widely applied in industry. In this study, based on castor bean reference genome, three RcWRIs genes (RcWRI1, RcWRI2 and RcWRI3) were identified and the expressed association of RcWRI1 with oil accumulation were determined. Heterologous transformation of RcWRI1 significantly increased oil content in tobacco leaf, confirming that RcWRI1 activate lipid biosynthesis pathway. Using DNA Affinity Purification sequencing (DAP-seq) technology, we confirmed RcWRI1 binding with Transcription Start Site of genes and identified 7961 WRI1-binding candidate genes. Functionally, these identified genes were mainly involved in diverse metabolism pathways (including lipid biosynthesis). Three cis-elements AW-box ([CnTnG](n)7[CG]) and AW-boxes like ([GnAnC](n)6[GC]/[GnAnC](n)7[G]) bound with RcWRI1 were identified. Co-expression network analysis of RcWRI1 further found that RcWRI1 might be widely involved in biosynthesis of storage materials during seed development. In particular, yeast one hybrid experiments found that both AP2 domains within RcWRI1 were required in binding targeted genes. These results not only provide new evidence to understand the regulatory mechanism of RcWRI1 in regulation of oil accumulation during castor bean seed development, but also give candidate gene resource for subsequent genetic improvement toward increasing oil content in oilseed crops.

关键词: Castor bean, WRI transcription factor, Oil accumulation, Developing seeds, Lipid gene

Abstract: The transcription factor WRINKLED1 (WRI1), a member of AP2 gene family that contain typical AP2 domains, has been considered as a master regulator regulating oil biosynthesis in oilseeds. However, the regulatory mechanism of RcWRI1 in regulating oil accumulation during seed development has not been clearly addressed. Castor bean (Ricinus communis) is one of the most important non-edible oil crops and its seed oils are rich in hydroxy fatty acids, widely applied in industry. In this study, based on castor bean reference genome, three RcWRIs genes (RcWRI1, RcWRI2 and RcWRI3) were identified and the expressed association of RcWRI1 with oil accumulation were determined. Heterologous transformation of RcWRI1 significantly increased oil content in tobacco leaf, confirming that RcWRI1 activate lipid biosynthesis pathway. Using DNA Affinity Purification sequencing (DAP-seq) technology, we confirmed RcWRI1 binding with Transcription Start Site of genes and identified 7961 WRI1-binding candidate genes. Functionally, these identified genes were mainly involved in diverse metabolism pathways (including lipid biosynthesis). Three cis-elements AW-box ([CnTnG](n)7[CG]) and AW-boxes like ([GnAnC](n)6[GC]/[GnAnC](n)7[G]) bound with RcWRI1 were identified. Co-expression network analysis of RcWRI1 further found that RcWRI1 might be widely involved in biosynthesis of storage materials during seed development. In particular, yeast one hybrid experiments found that both AP2 domains within RcWRI1 were required in binding targeted genes. These results not only provide new evidence to understand the regulatory mechanism of RcWRI1 in regulation of oil accumulation during castor bean seed development, but also give candidate gene resource for subsequent genetic improvement toward increasing oil content in oilseed crops.

Key words: Castor bean, WRI transcription factor, Oil accumulation, Developing seeds, Lipid gene

Qing Tan, Bing Han, Mohammad Enamul Haque, Ye-Lan Li, Yue Wang, Di Wu, Shi-Bo Wu, Ai-Zhong Liu. The molecular mechanism of WRINKLED1 transcription factor regulating oil accumulation in developing seeds of castor bean[J]. Plant Diversity, 2023, 45(04): 469-478.

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The molecular mechanism of WRINKLED1 transcription factor regulating oil accumulation in developing seeds of castor bean

The molecular mechanism of WRINKLED1 transcription factor regulating oil accumulation in developing seeds of castor bean

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