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Plant Diversity ›› 2009, Vol. 31 ›› Issue (04): 326-334.DOI: 10.3724 SP.J.1143.2009.09033

• 研究论文 • 上一篇    下一篇


刘润华1 , 2 , 江文波1 , 2 , 余迪求1   

  1. 1 中国科学院西双版纳热带植物园, 云南昆明 650223 ; 2 中国科学院研究生院, 北京 100049
  • 收稿日期:2009-02-23 出版日期:2009-08-25 发布日期:2009-08-25
  • 通讯作者: 余迪求

The Response of AtCER to Oxidative Stress in Arabidopsis thaliana

LIU Run-Hua1 , 2 , JIANG Wen-Bo1 , 2 , YU Di-Qiu1   

  1. 1 Xishuangbanna Tropical Botanical Garden , Chinese Academy of Sciences, Kunming 650223 , China;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049 , China
  • Received:2009-02-23 Online:2009-08-25 Published:2009-08-25
  • Contact: YU Di-Qiu

摘要: 以拟南芥哥伦比亚生态型(Col ) 和神经酰胺酶突变体为实验材料, 通过对突变体的一系列生理生化指标的测定, 来研究拟南芥神经酰胺酶基因( AtCER) 对H2O2 的响应。利用PCR 和Northern blot 获得了9 个AtCER 纯合单突变体。不同浓度H2 O2 处理野生型和突变体后, 发现突变体对H2O2 的反应比野生型更加敏感。H2O2 处理后突变体叶片出现比野生型更严重的黄化现象和坏死斑点, 总叶绿素含量也比野生型下降的更快, 电导率测定也发现突变体比野生型的电导率增加得更多。抗氧化酶活性的分析结果发现H2O2 处理后, 突变体的抗氧化酶活性比野生型提高了1.5~3 倍。上述研究结果说明AtCER 参与了H2O2诱导的氧化胁迫反应。

关键词: 拟南芥, 神经酰胺, 神经酰胺酶, H2O2

Abstract: Ceramide is a second messenger involved in many significant signal pathways of plants, such as cell growth, reduplication, differentiation, senescence and apoptosis . This work investigated the AtCER function in oxidative stress caused by H2O2 . The leaves of AtCER mutants cer1 , cer2 and cer3 , exhibited more severe levels of yellowing and necrotic lesions
than wild- type with H2O2 treatment . Further investigation by the physiological and biochemical methods, indicated that chlorophyll content of these mutants decreased more quickly than do wild type plants, and their conductivity increased significantly , and the antioxidases activity in leaves of AtCER mutants were approximately 1.5 or 3-fold higher than that inwild - type after H2O2 treatment . All of those results suggested that AtCER may involved in H2O2 -induced Oxidative stress.

Key words: Arabidopsis thaliana