[an error occurred while processing this directive]

Plant Diversity ›› 2009, Vol. 31 ›› Issue (02): 166-172.DOI: 10.3724 SP.J.1143.2009.08196

• 研究论文 • 上一篇    下一篇

外源一氧化氮供体硝普钠对红树植物桐花树气孔运动的调控效应

肖 强1 , 2 , 林晓梅2 , 吴飞华2 , 陈 娟2 , 郑海雷2   

  1. 1 湖北省生物资源保护与利用重点实验室、湖北民族学院, 湖北恩施 445000;
    2 厦门大学生命科学学院, 福建厦门 361005
  • 收稿日期:2008-10-27 出版日期:2009-04-25 发布日期:2009-04-25
  • 通讯作者: 郑海雷

Effect of Exogenous Nitric Oxide Donor Sodium Nitroprusside on Stomatal Movement in Leaves of Mangrove Plant Aegiceras corniculatum

XIAO Qiang1 , 2 , LIN Xiao-Mei2 , WU Fei-Hua2 , CHEN Juan2 , ZHENG Hai-Lei2   

  1. 1 Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, Enshi 445000 , China ; 2 School of Life Sciences, Xiamen University , Xiamen 361005 , China
  • Received:2008-10-27 Online:2009-04-25 Published:2009-04-25
  • Contact: ZHENG Hai-Lei

摘要: 探索一种用材简单、操作方便、真实性强的观察红树植物桐花树叶片气孔的制片技术, 并利用该技术研究不同浓度、不同处理时间的一氧化氮(NO) 供体硝普钠( sodium nitroprusside, SNP) 对桐花树气孔开闭的影响, 探讨了NO 调控的气孔运动与外源Ca2 + 的关系以及NO 与H2O2 在调节气孔运动过程中的关系。结果表明: 在搅碎法、指甲油印迹法、牛皮胶印迹法三种观察气孔方法中, 牛皮胶印迹法是观察气孔开度变化的最佳方法。NO 能够诱导桐花树气孔快速关闭, 且表现出明显的时间效应与浓度效应。NO 导致的气孔关闭与Ca2+ 的参与有密切关系, NO 与H2O2 存在明显的协同效应, 可以促进气孔关闭。

关键词: 一氧化氮, 桐花树, 气孔, 钙, H2O2

Abstract: The aim of the present paper was to find out a simple and easy- to-go method to observe the stomatal movement of a mangrove plant Aegiceras corniculatum . The new method was used to study the following areas, inculing the effect of exogenous nitric oxide (NO) on the leaf stomatal movement under different concentrations and different treated time , the
effect of Ca2+ in the regulation of stomatal movement induced by exogenous NO, the interactional effect between NO and H2O2 in the regulationb of stomatal movement. The results suggested that the oxhide pastern print was the best way to observe stomatal movement of A. corniculatum compared with the blender method and the nail enamel print method. The stomatal
closure induced by NO was associated with Ca2+ in dose dependent manner. It was also proved that a rapid stomatal closure could be co- induced by NO and H2O2 in A. corniculatum guard cells .

Key words: Nitric oxide

中图分类号: