拟南芥黄化突变体chlm-4的基因定位与分析

Plant Diversity ›› 2010, Vol. 32 ›› Issue (02): 134-140.

拟南芥黄化突变体chlm-4的基因定位与分析

曹志林1,2, 林冠男1, 丁文庆1, 杨仲南1, 崔永兰1

1 上海师范大学生命与环境科学学院，上海200234；2 河南工程学院资源与环境工程系，河南郑州451191

收稿日期:2009-11-06 出版日期:2010-04-25 发布日期:2010-06-29
通讯作者: 崔永兰

Mapping and Analysis of a Yellow Mutant chlm-4 of Arabidopsis thaliana (Cruciferae)

CAO ZhiLin1,2, LIN GuanNan1, DING WenQing1, YANG ZhongNan1, CUI YongLan1

1 College of Life and Environment, Shanghai Normal University, Shanghai 200234, China;2 Department of Resource and Environmental Engineering, Henan Institute of Engineering, Zhengzhou 451191, China

Received:2009-11-06 Online:2010-04-25 Published:2010-06-29
Contact: CUI YongLan

摘要/Abstract

摘要： 叶绿素是光合作用必需的重要色素，其合成需要Mg原卟啉Ⅸ甲基转移酶等一系列酶的催化。我们通过筛选拟南芥EMS（乙基磺酸甲酯，ethylmethane sulphonate）突变体库，分离到一个黄化突变体。该突变体叶绿素含量显著减少，叶绿体内垛叠的基粒缺失。遗传学分析表明，该突变体的黄化表型是由单基因控制的隐性性状。利用图位克隆的方法最终将基因定位在第IV条染色体分子标记F13M23和T30C3之间114 kb的区间内，其中包含编码Mg原卟啉Ⅸ甲基转移酶的CHLM基因。通过测序及等位分析表明该突变体是chlm的等位突变体，命名为chlm4。在chlm4中，CHLM蛋白的Gly59突变成Glu59，说明Gly59对于Mg原卟啉Ⅸ甲基转移酶功能的行使是必需的。

关键词: 拟南芥；CHLM；等位分析；图位克隆

Abstract: Chlorophylls are essential for photosynthesis. Chlorophyll biosynthesis is catalyzed by a series of enzyme complexes, such as Mgprotoporphyrin IX methyltransferase. A yellow mutant of Arabidopsis was isolated using an enthylmethane sulfonate (EMS) mutagenesis strategy. Chlorophyll content dramatically reduced and grana stacking was absent in the mutant. Genetic analysis indicated that the mutant was controlled by a single recessive gene. Using mapbased cloning strategy, the gene responsible for the mutant phenotype was mapped to a region of 114 kb between the molecular markers F13M23 and T30C3 on chromosome 4, in which the CHLM gene encoding Mgprotoporphyrin IX methyltransferase was included. The mutant was proved to be an allelic mutant of CHLM gene by sequencing and allelism test and then was designated as chlm4. Gly59 of CHLM was replaced by Glu59 in chlm4, which indicated that Gly59 was essential for the function of Mgprotoporphyrin IX methyltransferase.

Key words: Arabidopsis thaliana

中图分类号:

Q 78

曹志林1,2, 林冠男1, 丁文庆1, 杨仲南1, 崔永兰1 . 拟南芥黄化突变体chlm-4的基因定位与分析[J]. Plant Diversity, 2010, 32(02): 134-140.

CAO ZhiLin, LIN GuanNan, DING WenQing, YANG ZhongNan, CUI YongLan. Mapping and Analysis of a Yellow Mutant chlm-4 of Arabidopsis thaliana (Cruciferae)[J]. Plant Diversity, 2010, 32(02): 134-140.

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