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Plant Diversity ›› 2010, Vol. 32 ›› Issue (02): 147-150.DOI: 10.3724/SP.J.1143.2010.09204

• 研究论文 • 上一篇    下一篇

快速碱化因子基因BcRALF在菜心中的克隆及序列分析

李焰焰1,2，曹家树2   

  1. 1 阜阳师范学院生命科学学院，安徽 阜阳236041； 2 浙江大学蔬菜研究所细胞与
    分子生物学实验室，浙江 杭州310029
  • 收稿日期:2009-10-14 出版日期:2010-04-25 发布日期:2010-06-29
  • 通讯作者: 曹家树

Isolation and Molecular Characterization of RALFLike Gene BcRALF from Brassica campestris ssp.chinensis var.parachinensis

LI YanYan1, 2, CAO JiaShu2   

  1. 1 School of Life Sciences, Fuyang Teachers College, Fuyang 236041, China; 2 Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University , Hangzhou 310029, China
  • Received:2009-10-14 Online:2010-04-25 Published:2010-06-29
  • Contact: CAO JiaShu

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摘要： 快速碱化因子是近年来新发现的一种植物多肽类信号分子，广泛存在于高等植物中。通过已得到的普通白菜的快速碱化因子基因 BcMF14（GenBank序列登录号EF523516）的核苷酸序列，在其编码框两侧设计引物，从菜心中克隆出该类信号分子基因，命名为BcRALF (登陆号：GU086228)。序列同源比对表明该基因与花椰菜、拟南芥等的快速碱化因子基因有很高的相似性，证明BcRALF属于快速碱化因子家族。蛋白质特征预测以及蛋白序列结构分析发现BcRALF蛋白包含有多个生物活性位点，符合其作为多肽类信号分子类蛋白的特征。

关键词: 菜心；BcRALF；快速碱化因子

Abstract: The rapid alkalinization factor (RALF) gene is a new found plant polypeptide signal molecule, wide spreading in higher plants. In this study BcRALF was cloned from Brassica campestris sspchinensis Makino varparachinensis based on BcMF14 (GenBank accession number EF523516) from Bcampestris ssp. chinensis var. communis cv. Aijiaohuang. The sequence of this gene was 273 bp (GU086228) and was identified as a rapid alkalization factor gene according to its high identity with Boleracea var botrytis BoRALF1 and Arabidopsis thaliana RALFL9. Protein characteristics and sequence structure were predicted, and moreover, many bioactive sites were found. The results showed that the characteristics of the BcRALF protein consistent with its category as a peptide signal molecule.

Key words: Brassica campestris sspchinensis varparachinensis

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