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Plant Diversity ›› 2011, Vol. 33 ›› Issue (2): 201-208.DOI: 10.3724/SP.J.1143.2011.10183

• 研究论文 • 上一篇    下一篇

转座子插入宿主基因在稻属中的分布式样及其适应意义的初探

 樊亚宇1、2,  高立志1     

  1. 1 中国科学院昆明植物研究所植物种质资源与基因组学研究中心,云南 昆明  650204;2 中国科学院研究生院,北京  100049
  • 收稿日期:2010-10-24 出版日期:2011-04-25 发布日期:2011-01-05
  • 基金资助:

    中国科学院引进国外杰出人才计划 (CAS), 云南省引进高端人才项目 (20080A009), 中国科学院知识创新工程方向性重点项目 (KSCX2-YW-N-029), 国家“973”重大项目子专题 (973 Program 2007CB815703) 及中国科学院昆明植物研究所人才引进启动项目 (to L.Z. GAO)

A Preliminary Study on Patterns of TE-gene Associations in Oryza (Poaceae) and Adaptive Significance

 FAN  Ya-Yu-1、2,   Gao-Li-Zhi-1   

  1. 1 Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2010-10-24 Online:2011-04-25 Published:2011-01-05

摘要:

转座子在各类真核生物基因组中都占有很高的比例,它们对宿主基因组特别是关联的基因在结构、功能和进化上都起着重要的作用。基于生物信息学分析,本研究选择了水稻基因组中2个被转座子插入的宿主基因,通过PCR扩增和琼脂糖凝胶电泳分析,获得了转座子在稻属16个代表物种94份材料中的插入式样。结果表明,这2个转座子在稻属中的分布式样与插入时间不同,基因LOC_Os02g26349中的转座子在AA-基因组的物种中全部存在,基因LOC_Os02g45130中的转座子则插入稻属AA-基因组的部分物种中,与AA-基因组的物种的系统发育关系相吻合。转座子在宿主基因组中不同的分布与保留式样以及插入后已经固定在不同地理来源的群体中,暗示了它们在物种进化过程中对宿主基因可能存在适应性意义。

关键词: 转座子, 稻属, AA-基因组, 插入

Abstract:

Transposable elements (TEs) have been found to be significant fractions of eukaryotic genomes. Moreover, they make great contributions to the structure, function and evolution of host genomes and associated genes in particular. Starting from bioinformatics searches, this study investigated the distribution of two TE-gene associations in 94 strains belonging to 16 Oryza species through PCR and agarose gel electrophoresis. We found that TE-insertions in the gene LOC_Os02g26349 distributed in AA-genome species while TE inserted into the gene LOC_Os02g45130 in part of AA-genome species. The result is obviously in agreement with the phylogeny of AA-genome species. The observed patterns of TE-gene associations in Oryza species and the fixation of TE-insertions within populations of different geographic origins together imply adaptive significance of TEs in the evolution of their host genes and genomes.

Key words: Transposable element, Oryza, AA-genome group, Insertion

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