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Plant Diversity ›› 2013, Vol. 35 ›› Issue (6): 743-750.DOI: 10.7677/ynzwyj201313199

• 研究论文 • 上一篇    下一篇


 张玉霄1、2, 许宇星4, 马朋飞1、2, 张丽娜1、2、3, 李德铢1、2   

  1. 1 中国科学院昆明植物研究所生物多样性与生物地理学重点实验室,云南 昆明650201; 2 中国科学院
    昆明植物研究所中国西南野生生物种质资源库,云南 昆明650201; 3 中国科学院大学,
    北京100049;4 西北农林科技大学生命科学学院,陕西 杨凌712100
  • 收稿日期:2013-09-30 出版日期:2013-11-25 发布日期:2013-10-14
  • 基金资助:

    国家自然科学基金 (31100148);NSFC云南联合基金 (U1136603);科技部科技基础性工作专项项目 (2013FY112600);科技部国家高技术研究发展计划 (863计划) 主题项目 (2012AA021801);中国科学院大科学装置项目 (2009-LSFGBOWS01)

Selection of Potential Plastid DNA Barcodes for Bambusoideae (Poaceae)

 ZHANG  Yu-Xiao-1、2, HU  Yu-Xing-4, MA  Peng-Fei-1、2, ZHANG  Li-Na-1、2、3, LI  De-Zhu-1、2   

  1. 1 Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650201, China; 2 Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy
    of Sciences, Kunming 650201, China; 3 University of Chinese Academy of Sciences, Beijing 100049, China;
    4 College of Life Sciences, Northwest A & F University, Yangling 712100, China
  • Received:2013-09-30 Online:2013-11-25 Published:2013-10-14



关键词: 竹亚科, DNA条形码, 插入/缺失, 分子系统学, 叶绿体片段筛选


 Bamboos are a taxonomically difficult group. DNA barcoding provides another supplementary method for the bamboo identification. The core DNA barcodes (rbcL+matK) have quite low discriminatory power in bamboos, therefore, it is crucial to select effective DNA barcodes for bamboos. We scanned the entire chloroplast genomes that were published in Bambusoideae in order to search highly variable regions. Sixteen regions were selected including one coding region and 15 intergenic spacers, and the universality, sequencing success rates, and the variable characters were testified in 30 taxa of 22 species in 10 genera of Arundinarieae and Bambuseae. The results indicated that (1) the amplification success rates were high for each region, but there were some problems in sequencing for some regions due to polystructures; (2) indels should be coded as informative characters; (3) the region trnGtrnT(t) was the most variable one among the 16 regions and it was suggested as the prior candidate DNA barcode in Bambusoideae; (4) the combination “trnGtrnT(t)+X” can be used in DNA barcoding and molecularphylogeny reconstruction study of bamboos.

Key words:  Bambusoideae, DNA barcoding, Indels, Molecular phylogeny, Plastid region selection