钾素对药用植物短葶飞蓬生长和有效成分积累的影响

doi:10.3724/SP.J.1143.2011.10215

摘要/Abstract

摘要：

在不同钾素供应下对药用植物短葶飞蓬（Erigeron breviscapus）进行了人工栽培试验，探讨了钾素对其生长和次生代谢产物积累的影响。结果显示：在0~0.372g/株钾素施用量范围内，钾素供应量与短葶飞蓬单株生物量以及总咖啡酸酯和灯盏乙素的产量呈极显著的正相关；但植株总咖啡酸酯和灯盏乙素的含量则随钾素供应量增加先升高后降低；总咖啡酸酯和灯盏乙素含量与植株N含量呈显著的线性负相关，与碳素/营养平衡假说一致。0~0.248g/株钾素施用量范围内，短葶飞蓬地上生物量和总生物量与总咖啡酸酯和灯盏乙素含量都呈现出极显著的正相关。研究表明：当钾供应量不十分丰富时，适当增施钾可促进植株酚类次生代谢产物的积累，但高钾供应量则抑制积累。并不是只有在生长胁迫生境中才能得到高的酚类次生代谢产物积累，生长与次生代谢产物积累间不存在权衡关系。

关键词: 灯盏花, 次生代谢, 生物量, 钾

Abstract:

Erigeron breviscapus a medicinal plant was planted under different potassium levels to investigate effects of potassium on the growth and the accumulation of secondary metabolites, address the relationship between them in medicinal plants in the plantation. Results showed: In 0-0.372g/plant potassium levels, the biomass of E.breviscapus and yields of 2 active constituents, total caffeates and scutellarin, were significantly positively correlated with the amount of K fertilizer. Contents of total caffeates and scutellarin were significantly positively correlated with the amount of K fertilizer from 0 to 0.248g/plant, but negatively correlated with the amount of K fertilizer from 0.248 to 0.372g/plant. Contents of total caffeates and scutellarin were negatively correlated with contents of N in E.breviscapus, as predicated by the carbon/nutrient balance hypothesis. The findings suggest that: In condition of low potassium availability in soil more potassium fertilizer is possible to increase flavonoids accumulation in plant, but in high potassium availability is to reduce. Flavonoids accumulation in plants is not only found to increase in stress environment, but also in normal environment. There is not a tradeoff between the growth and accumulation of secondary metabolites in plant.

Key words: Erigeron breviscapus, Secondary metabolites, Biomass, Potassium

中图分类号:

Q 945

苏文华, 张光飞, 郭晓荣, 周睿. 钾素对药用植物短葶飞蓬生长和有效成分积累的影响[J]. Plant Diversity, 2011, 33(4): 396-402.

SU Wen-Hua, ZHANG Guang-Fei, GUO Xiao-Rong, ZHOU Rui. Effects of Potassium on the Growth and Accumulation of Active Constituents of a Medicinal Plant Erigeron breviscapus (Asteraceae)[J]. Plant Diversity, 2011, 33(4): 396-402.

参考文献

国家药典委员会, 2005. 中国药典第一部［M］. 北京: 化学工业出版社, 447—448
许海琴, 许列琴, 许江苇, 2003. 常用天然提取物质量标准参考手册［M］. 北京: 化学工业出版社和现代生物技术与医药科技出版中心, 124—125
廖红, 严小龙, 2003. 高级植物营养学［M］. 北京: 科学出版社,156—171
Andrew RL, Wallis IR, Harwood CE et al., 2007. Heritable variation in the foliar secondary metabolite sideroxylonal in Eucalyptus confers crossresistance to herbivores［J］. Oecologia, 153 (4): 891—901
Bazzaz FS, Nona RC, Coley PD et al., 1987. Allocating resources to reproduction and defense［J］. Bioscience, 37: 53—67
Bhaskar CV, Rao GR, Reddy KB, 2001. Effect of nitrogen and potassium nutrition on sheath rot incidence and phenol content in rice (Oryza sativa L.)［J］. Indian Journal of Plant Physiology, 16 (3): 254—257
Bryant JP, Chapin FS, Klein DR, 1983. Carbon/nutrient balance of boreal plants in relation to vertebrate herbivory［J］. Oikos, 40: 357—368
Chapin FS III, Aronld JB, Christopher BF et al., 1987. Plant response to multiple environmental factors［J］. Bioscience, 37: 49—77
Coley PD, Brant JP, Chapin FS,1985. Resource availability and plant antiherbivore defense［J］. Science, 230: 895—899
Hamilton JG, Zangerl AR, DeLucia EH et al., 2001. The carbonnutrient balance hypothesis: its rise and fall［J］. Ecology Letters, 4: 86—95
Hring DA, Huber MJ, Suter D et al., 2008. Plant enemyderived elicitors increase the foliar tannin concentration of Onobrychis viciifolia without a tradeoff to growth［J］. Annals of Botany, 102 (6): 979—987
Herms DA, Masttson WJ, 1992. The dilemma of plants: to grow or to defend［J］. Quarterly Review of Biology, 67: 283—335
Huang LQ (黄璐琦), Guo LP (郭兰萍), 2007. Secondary metabolites accumulating and geoherbs formation under enviromental stress［J］. China Journal of Chinese Materia Medica (中国中药杂志), 32(4): 277—280
Frischknecht PM, Schuhmacher K, Mullerscharer H et al., 2001. Phenotypic plasticity of Senecio vulgaris from contrasting habitat types: growth and pyrrolizdine alkaloid formation［J］. Journal of Chemical Ecology, 27(2): 343—358
Kong CH (孔垂华), Xu T (徐涛), Hu F (胡飞) et al., 2000. Allelopathy under environmental stress and its induced mechanism［J］. Acta Ecologica Sinica (生态学报), 20: 849—854
Koricheva J, 2002. The carbonnutrient balance hypothesis is dead; long live the carbonnutrient balance hypothesis?［J］. Oikos, 98: 537—539
Li XH (李秀华), Su WH (苏文华), Zhou H(周鸿) et al., 2009. Effects of doubled CO2 concentration on Erigeron breviscapus growth and its active constituent accumulation［J］. Chinese Journal of Applied Ecology (应用生态学报), 20 (8) : 1852—1856
Li XH (李絮花), Yang SX (杨守祥), 2002. The effects of K fertilizing on N and K contents in tobacco leaves［J］. Acta Tabacaria Sinica (中国烟草学报), 8 (3): 17—25
McArthur C, Marsh NR, Close DC et al., 2003. Nursery conditions affect seedling chemistry, morphology and herbivore preference for Eucalyptus nitens［J］. Forest Ecology and Management, 176: 585—594
Mondy NI, Munshit CB, 1993. Effect of type of potassium fertilizer on enzymatic discoloration and phenolic, ascorbic acid, and lipid contents of potatoes［J］. Journal of Agricultural and Food Chemistry, 41: 849—852
Montanari M, Degl′Innocenti E, Maggini R et al., 2008. Effect of nitrate fertilization and saline stress on the contents of active constituents of Echinacea gustifolia DC［J］. Food Chemistry, 107: 1461—1466
Moore BD, Wallis IR, Wood JT et al., 2004. Foliar nutrition, site quality, and temperature influence foliar chemistry of tallowwood (Eucalyptus microcorys)［J］. Ecological Monographs, 74(4): 553—568
Stewart AJ, Chapman W, Jenkins GI et al., 2001. The effect of nitrogen and phosphorus deficiency on flavonol accumulation in plant tissues［J］. Plant, Cell and Environment, 24: 1189—1197
Su WH (苏文华), Zhang GF (张光飞), Li XH (李秀华) et al., 2005. Relationship between accumulation of secondary metabolism in medicinal plant and environmental condition［J］. Chinese Traditional and Herbal Drugs (中草药), 36 (9): 1415—1418
Su WH (苏文华), Zhang YN (张亚妮), Zhang GF (张光飞) et al., 2009a. Effect of soil types on contents of scutellarin and caffeates in Erigeron breviscapus［J］. Journal of Wuhan Botanical Research(武汉植物学研究), 27（4）：407—411
Su WH (苏文华), Zhang GF (张光飞), Zhou H (周鸿) et al., 2009b. Effects of nitrogen availability in soil on contents of flavonoids and caffeates in a medicinal plant, Erigeron breviscapus［J］. Acta Phytoecologica Sinica（植物生态学报），33 (5): 885—892
Su WH (苏文华), Zhang GF (张光飞), Wang ZM (王泽民) et al., 2009c. Effect of N, P and K fertilizers on growth of Erigeron breviscapus and its active constituent accumulation［J］. Chinese Traditional and Herbal Drugs (中草药), 40 (12): 1963—1966
Su WH (苏文华), Zhang GF (张光飞), Zhou H (周鸿) et al., 2010a. Spatial variability in the content of scutellarin and caffeates of Erigeron breviscapus［J］. Acta Ecologica Sinica (生态学报), 30 (4): 1109—1116
Su WH (苏文华), Zhang GF (张光飞), Zhou H (周鸿) et al., 2010b. Effects of nitrogen on the growth and accumulation of active constituents of a medicinal plant Erigeron breviscapus［J］. Journal of Yunnan Botanical Research (云南植物研究), 32 (1): 41—46
Walls R, Appel H, Cipollini M et al., 2005. Fertilty, root reserves and the cost of inducible defenses in the perennial plant Solanum carolinense［J］. Journal of Chemical Ecology, 31: 2263—2288
Vance CP, UhdeStone C, Allan DL, 2003. Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource［J］. New Phytologist, 157: 423—447
Zhang YN (张亚妮), Su WH (苏文华), Zhang GF (张光飞), 2009. Effect of water stress on content of active constituents in Erigeron breviscaps［J］. China Journal of Chinese Materia Medica (中国中药杂志), 34 (10): 1191—1194
Zhang Y (张燕), Liu Y (刘勇), Wang WQ (王文全) et al., 2007. Effects of N P K on yield and baicalin contents of Scutellaria baicalensis［J］. Journal of Chinese Medicinal Materials (中药材), 30 (4): 286—387

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