毛竹对大气臭氧浓度倍增的生理响应

doi:10.3724/SP.J.1143.2012.11088

Plant Diversity ›› 2012, Vol. 34 ›› Issue (01): 69-75.DOI: 10.3724/SP.J.1143.2012.11088

毛竹对大气臭氧浓度倍增的生理响应

庄明浩1，李迎春1，李应1，顾李俭2，陈双林1

1 中国林业科学研究院亚热带林业研究所，浙江富阳311400; 2 杭州临安太湖源
观赏竹种园有限公司，浙江临安311306

收稿日期:2011-06-02 出版日期:2012-02-25 发布日期:2012-04-24
基金资助:
国家林业公益性行业科研专项(201004008)；中央级公益性科研院所基本科研业务费专项资金(RISF6915)；浙江省省院合作项目(2010SY01)

The Physiological Response of Phyllostachys edulis under the Elevated Atmospheric Ozone

PENG Ming-Hao-1, LI Ying-Chun-1, LI Ying-1, GU Li-Jian-2, CHEN Shuang-Lin-1

1 Research Institute of Subtropical Forestry, Chinese Forestry Academy, Fuyang 311400 China;
2 Hangzhou Lin’an Taihuyuan Landscaping Bamboo Garden, Lin’an 311306, China

Received:2011-06-02 Online:2012-02-25 Published:2012-04-24
Supported by:
国家林业公益性行业科研专项(201004008)；中央级公益性科研院所基本科研业务费专项资金(RISF6915)；浙江省省院合作项目(2010SY01)

摘要/Abstract

摘要：

为了给气候变化背景下的竹林培育应对策略提供理论依据，以毛竹 (Phyllostachys edulis) 为材料，运用开顶式气室 (OTCs) 模拟当前环境背景大气O3浓度（NF，40~45nL·L-1）的倍增1倍（TR1，92~106nL·L-1）和倍增2倍（TR2, 142~160nL·L-1）情景，分析叶片光合色素、脂质过氧化及抗氧化酶等生理指标的变化规律。结果表明：大气O3浓度倍增处理90d时，毛竹叶片Chl、Car含量和SOD活性显著降低，叶片相对电导率和可溶性蛋白、MDA、O2-含量及POD活性显著升高。叶片O2-含量与SOD活性、光合色素含量呈极显著负相关，而与叶片相对电导率、POD活性和MDA、可溶性蛋白含量呈显著正相关。研究表明大气O3浓度倍增会导致毛竹叶片老化加快，光合色素降解或合成受阻，膜脂过氧化，膜结构和氧化系统破坏，影响毛竹的正常生长。本文从抗性生理方面揭示了毛竹对大气臭氧胁迫的耐受性，为竹子应对气候变化研究提供了参考。

关键词: 毛竹, 臭氧, 光合色素, 膜脂过氧化, 抗氧化酶

Abstract:

In order to provide theoretical evidences for bamboo cultivation under the background of climate change, the method of opentop chambers (OTCs) was employed to approach the effects of elevated ozone on photosynthetic pigments content, lipid peroxidation and antioxidation enzymes in the leaves of Phyllostachys edulis. The result indicated that with the increasing of ozone concentration, the content of the chlorophyll, carotenoid and SOD activity decreased, while the content of souble protein, relative electrical conductivity, contents of MDA, O2- and the activity of POD increased.Correlation analyses showed that there was significant negative correlation between O2- content and SOD activity as well as photosynthetic pigment,whereas significant positive correlation between O2- and relative electeical conductivity, MDA, POD as well as soluble protein. It was summarried that, on one hand, long time exposure to high ozone concentration exposure, accelerated the aging of leaves, intensified the damage to membrane lipid, and destroyed the structure of cell membrane and the function of antioxidation system as well. It affected the regular growth of P.edulis. This research was conducted to provide theoretical evidence for bamboo cultivation during this time of possible climate change.

Key words: Phyllostachys edulis, Ozone, Photosynthetic pigment, Membrane lipid peroxidation, Antioxidant enzymes

中图分类号:

Q 945

庄明浩1, 李迎春1, 李应1, 顾李俭2, 陈双林1. 毛竹对大气臭氧浓度倍增的生理响应[J]. Plant Diversity, 2012, 34(01): 69-75.

PENG Ming-Hao-, LI Ying-Chun-, LI Ying-, GU Li-Jian-, CHEN Shuang-Lin. The Physiological Response of Phyllostachys edulis under the Elevated Atmospheric Ozone[J]. Plant Diversity, 2012, 34(01): 69-75.

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毛竹对大气臭氧浓度倍增的生理响应

The Physiological Response of Phyllostachys edulis under the Elevated Atmospheric Ozone

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