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Plant Diversity ›› 2012, Vol. 34 ›› Issue (3): 211-221.DOI: 10.3724/SP.J.1143.2012.12041

• 研究论文 •    下一篇


 丁铭, 曾丽萍, 马红, 张宁   

  1. 复旦大学生命科学院,植物科学研究所,进化生物学中心,上海200433
  • 收稿日期:2012-03-26 出版日期:2012-06-25 发布日期:2012-05-04
  • 基金资助:

    国家自然科学基金委资助项目 (31100156);中国博士后基金资助项目 (20100480549, 201003241)

The Use of Lowcopy Nuclear Genes for Reconstructing the Phylogeny of Lowlevel Taxonomic Hierarchies: Evidence from Brassicaceae

 DING  Ming, ZENG  Li-Ping, MA  Hong, ZHANG  Ning   

  1. Institute of Plant Biology, Center for Evolutionary Biology, School of Life Sciences, Fudan University, Shanghai 200433, China
  • Received:2012-03-26 Online:2012-06-25 Published:2012-05-04



关键词: 分子系统学, 十字花科, 低拷贝核基因, MCM5, MLH1, AT3G54630, AT3G26730, AT2G47990


 Molecular systematics is widely used to determine organismal relationships. However, until now, systematics of angiosperms has mostly employed organellar genes, but these genes are generally inherited uniparentally, only partially reflecting the evolutionary history. In addition, chloroplast and mitochondrial genes are highly conserved, with limited numbers of phylogenetically informative characters. In contrast, nuclear genes are inherited biparentally and have relatively large numbers of informative sites, but only few nuclear genes have been utilized. In this work, sequences of five lowcopy nuclear genes were obtained in 17 species from Brassicaceae and used for phylogenetic analyses with the MaximumParsimony, MaximumLikelihood and Bayesian methods. Highly similar topologies were obtained using the five genes; in addition, compared with organellar genes, the nuclear genes had more phylogenetically informative sites and provided stronger supports. Specifically, the supporting values of all the nodes were 100% in MP trees using the five nuclear genes.Therefore,these five genes are excellent candidate marker genes for plant taxonomists who aim to resolve the relationships among lowlevel taxonomic hierarchies and might also be useful for DNA barcoding.

Key words: Molecular systematics, Brassicaceae, Low-copy nuclear genes, MCM5, MLH1, AT3G54630, AT3G26730, AT2G47990