珙桐种子休眠解除和萌发过程中主要抗氧化酶活性和代谢产物含量的变化

doi:10.7677/ynzwyj201515074

摘要/Abstract

摘要：

珙桐（Davidia involucrata Baill.）种子在休眠解除和萌发过程中，主要的抗氧化酶和代谢产物在有效清除活性氧和保证种子活力方面具有重要作用。研究以马边自然保护区采集的珙桐种子为研究对象，对其从休眠解除进入萌发阶段过程中的主要抗氧化酶的活性和代谢产物含量进行测定和分析。结果显示：层积初期，过氧化物酶（POD）、过氧化氢酶（CAT）、超氧化歧化酶（SOD）和谷胱甘肽还原酶（GR）活性均上升，但总抗氧化能力（TAOC）下降。可溶性蛋白质（Pr）下降而抗坏血酸（Vc）、游离氨基酸（FAA）含量增大；层积中期，SOD活性和TAOC呈先升高后下降的变化趋势。尽管CAT和GR活性下降，但POD活性保持较高的水平；层积后期，SOD和CAT活性较低，POD、GR活性和TAOC， FAA和Vc含量整体呈上升趋势；萌发阶段，各抗氧化酶活性均有显著增加 (P < 005)，Vc和FAA含量显著增加，Pr含量降低。研究结果表明，珙桐种子可以通过各抗氧化酶活性（POD, CAT, SOD和GR）和代谢产物（Pr, FAA和Vc）间的相互作用，在种子休眠解除和萌发过程中先后有效地消除活性氧对细胞结构完整性的影响，并促进珙桐种子的形态和生理后熟，从而保证种子顺利萌发。

关键词: 珙桐种子, 休眠, 萌发, 抗氧化酶, 代谢产物

Abstract:

The principal antioxidant enzymes and metabolites were reported to play a very important role in eliminating reactive oxygen species and maintaining vigour in dormancy breaking and germination of Davidia involucrate seeds. However, the researches on the change laws of content and activity are seldom. In present study, we chose Davidia involucrate seeds which collected from the Mabian Nature Reserve as experimental materials, and then investigated the activity of principal antioxidant enzymes and the content of metabolites in dormancy breaking and germination of the seeds. The results showed that the activity of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) exhibited an increasing trend but the activity of total antioxidant capacity (TAOC) decreased greatly in early stage of stratification. Comparing with the decrease of protein (Pr), the content of ascorbic acid (Vc) and free amino acid (FAA) significantly increased. In middle stage of stratification, the activity of SOD and TAOC increased firstly and then decreased at last. The activity of POD always maintained a higher level although the activity of CAT and GR decreased greatly. In terminal stage of stratification, the activities of POD, GR, TAOC and the content of FAA and Vc exhibited an increasing trend, while the activity of CAT and SOD maintained a low level. In germination stage, the activity of all antioxidant enzymes and the content of Vc and FAA were increased obviously (P < 005), but the content of the protein was decreased. These results suggested that Davidia involucrata seeds may have a good ability to efficiently remove the effects on cell structure caused by reactive oxygen species (ROS) by keeping the interaction of antioxidant enzymes and metabolites successively during the period of dormancy breaking and germination. This adjustment helps to ensure the germination of seeds by acceleration the postripening in morphological and physiological of progresses.

Key words: Davidia involucrata seeds, Dormancy, Germination, Antioxidant enzymes, Metabolites

中图分类号:

Q 945.3

杨艳1、2, 黎云祥1、2, 胥晓2、3. 珙桐种子休眠解除和萌发过程中主要抗氧化酶活性和代谢产物含量的变化[J]. Plant Diversity, 2015, 37(06): 779-787.

YANG Yan-, LI Yun-Xiang-, XU Xiao. The Activity of Principal Antioxidant Enzymes and the Content of Metabolites in Dormancy Breaking and Germination of Davidia involucrate Seeds[J]. Plant Diversity, 2015, 37(06): 779-787.

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