吸胀期低温处理对水青树种子萌发特性的影响

doi:10.7677/ynzwyj201515007

摘要/Abstract

摘要：

通过模拟野外环境条件的变化，研究吸胀期低温处理对水青树种子萌发的影响，以探讨限制水青树种群更新的影响因素。研究发现：（1）水青树种子的吸胀过程可以分为4个阶段，其吸水量将随土壤湿度的增加而增加，并在30%土壤湿度条件下基本达到充分吸胀状态；（2）随着野外埋藏时间的延长，水青树种子萌发率、发芽势及含水量均呈上升趋势，活力指数呈现先上升后逐渐下降的趋势；(3) 经不同低温处理的水青树种子，在20%或30%土壤湿度吸胀条件下，其萌发特性达到最高；（4）在20%土壤湿度条件下，低温处理2~4d或21~28d时，0℃最适于水青树种子的萌发；在30%土壤湿度条件下，低温处理14~28d时，-7℃最适于水青树种子的萌发；（5）在相同冷冻时间的条件下，随温度的降低，落叶中水青树种子的萌发率无显著变化；在相同低温条件下（0℃低温除外），种子的萌发率会在冷冻28d后有所提高。结果表明：一定范围内的吸胀期低温处理对水青树种子的萌发并无抑制作用，并不是限制水青树种群更新的主要因素。

关键词: 水青树, 种子萌发, 吸胀, 低温, 种群更新

Abstract:

In this paper, the effects of low temperature, soil moisture and freezing time on the germination of Tetracentron sinense Oliv. seeds were studied by simulating wild environment conditions, and the factors influencing the natural regeneration of Tsinense population were discussed. The results are as follows: (1) The imbibition process of Tsinense seed can be divided into four stages, the water absorbing capacity of Tsinense seed after imbibition would increase with the increase of soil moisture, and the seeds would be fully imbibed under the condition of 30% soil moisture; (2) The germination rate, germination force and water content of Tsinense seed increased with the increase of storage time, and the vigor index gradually decreased after increasing before; (3) At different low temperature, the germination rate of Tsinense seed reached the highest when the soil moisture is 20% or 30%; (4) When treated at 20% soil moisture for 2-4 days or 21-28 days, 0℃ is best for the germination rate of Tsinense seed; when treated at 30% soil moisture for 14-28 days, -7℃ is best fort he germination rate of Tsinense seed; (5) When treated at the same condition of freezing time, the germination rate of seeds in fallen leaves had no significant difference with the decrease of low temperature; under the same condition of low temperature (0℃ low temperature except), the germination rate increased after frozen 28 days. The results showed that there is no significant influence of low temperature during imbibition on germination characteristics of Tetracentron sinense seeds and indicating that low temperature during imbibition is not the main factor influencing the natural regeneration of Tsinense population.

Key words: Tetracentron sinense Oliv, Seed germination, Imbibition, Low temperature, Natural regeneration

中图分类号:

Q 945

憨宏艳, 许宁, 李珊, 张雪梅. 吸胀期低温处理对水青树种子萌发特性的影响[J]. Plant Diversity, 2015, 37(05): 586-594.

HAN Hong-Yan, XIU Ning, LI Shan, ZHANG Xue-Mei. The Effect of Low Temperature during Imbibition on Germination Characteristics of Tetracentron sinense (Tetracentraceae) Seeds[J]. Plant Diversity, 2015, 37(05): 586-594.

参考文献

傅家瑞, 1985. 种子生理［M］. 北京: 科学出版社, 21—78
马炜梁, 2009. 植物学［M］. 北京: 高等教育出版社, 101—108
杨弘远, 2009. 植物生殖［M］. 科学出版社, 40—49
吴征镒, 2004. 中国植物志 (第1卷) ［M］. 北京: 科学出版社, 125—126
Basking CC, Bskin JM, 2001. Seed: Ecology, Biogeography and Evolution of Dormancy and Germination［M］. San Diego: Academic Press, 5—45
Bowler C, Van M, 1922. Superoxide dismutase and stress tolerance［J］. Annual Review of Plant Physiology and Plant molecular Biology, 43: 83—116
Brewer SW, 2001. Lgnorant seed predators and factors affecting the seed survival of a tropical plant［J］. Oikos, 93: 32—41
Cao LL (曹玲玲), Gan XH (甘小洪), He S (何松), 2012. Effect of different geographical provenances and matrix on seed germination and seeding initial growth of Tetracentron Sinense［J］. Guihaia (广西植物), 32 (5): 656—662
Chang W (长伟), Wu JG (吴建国), Liu YH (刘艳红), 2010. Seed germination of three domain plant on Qilian mountain at different temperature and soil moisture［J］. Chinese Garden Abstract (中国园艺文摘), 4: 1—5
Chen JJ (陈娟娟), Du YF (杜凡杨), Yu M (宇明) et al., 2008. Study on community characteristics and protection of rare tree species Tetracentron sinense［J］. Joural of Southwest Forestry College (西南林学院学报), 28 (1): 13—22
Cindy Q, Ming CP et al., 2013. Population persistence of a Tertiary relict tree Tetracentron sinense on the ailao Mountains, Yunnan, China［J］. Journal Plant Research, 126: 651—659
Fu DZ, Bruce B, 2001. Tetracentron［A］// Wu ZY, Raven PH. Flora of China［M］. Beijing: Science Press; St. Louis: Missouri Botanical Garden Press, 6—125
Gan XH, Cao LL, Zhang X et al., 2013. Floral biology, breeding system and pollination ecology of an endangered tree Tetracentron sinense Oliv. (Trochodendraceae) ［J］ . Botanical Studies, 54—50
Hohengrin A, Gavieres, Mary TK et al., 2000. Seed germination response to cold stratification period and thermal regime in Phacelia secunda (Hydrophyllaceae) ［J］. Plant Ecology, 149: 1—8
Huang JY (黄金燕), Zhou SQ (周世强), Li RG (李仁贵), 2010. An elementary study of the ecological feature of Tetracentron sinense in wolong nature reserve［J］. Journal of Sichuan Forestry Science and Technology (四川林业科技), 31 (4): 72—75
Joe Wolf, 1978. Chiling injure in plants—the role of membrane lipid fluidity［J］. Plant Cell and Environment, 1 (4): 241—246
Li N (李宁), Bai B (白冰), Lu CH (鲁长虎), 2011. Recruitment limitation of Scutellaria plant population: from seed production to sapling establishment［J］. Acta Ecologia Sinica (生态学报), 31 (21): 6624—6630
Li WL (李文良), Zhang XP (张小平), Hao CY (郝朝运) et al., 2008. Characteristics of seed germination of the rare plant Cercidiphyllum japonicum［J］. Acta Ecologica Sinica (生态学报), 28 (11): 5445—5453
Li YY (李艳艳), 2008. NO resisting against chilling imbibition in tomato seeds［D］. Xian: Shaanxi Normal University (陕西师范大学), 7—9
Luo JD (罗靖德), Gan XH (甘小洪), Jia XJ (贾晓娟), 2010. Biological characteristics of seeds of Endangered plant Tetracentron sinense［J］. Acta Botanica Yunnanica (云南植物研究), 32 (8): 204—210
Lyons JM, 1973. Chiling injury in plants［J］. Anual Review of Plant Physiology, 24: 445—466
Niu SN (牛淑娜), 2012. Preliminary study on physiology and ecology of germination of Zostera marina L［D］. Seed, 2—3
Parrish DJ, Leopold AC, 1977. Transient changes during soybean imbibition［J］. Plant Physiology, 59: 1111—1115
Prasad TK, 1997. Role of catalase in inducing chilling tolerance in preemergent maize seedlings［J］. Plant Physiology, 114: 1369—1376
Ren JY (任坚毅), 2008. Study on natural regeneration and seed germination characteristics of Betula albo—sinensis at MT. Tai bai, China［D］. Xi’an: Northwest University (西北大学), 24—25
Shang HL (尚海琳), 2009. Geographic variation of morphology and physiology characteristics and seed germination Physiology of Sinopodophyllum hexandrum［D］. Xi’an: Northwest University (西北大学), 48—49
Santis A, Genchi G, 1999. Changes of mitochondrial properties in maize seedlings associated with selection for germination at low temperature ［J］. Plant Physiology, 119: 743—754
Simon EW, 1974. Phospholipids and Plant membrance permeability［J］. New Phytologist, 73: 377—420
Simon EW, 1972. RMraja harun.Leakage during seed imbibition［J］. Journal of Experimental Botany, 23: 1076—1085
Swift JG, 1973. Protein bodies, lipid layers and amyloplasts in freezeEtched peacotyledons［J］. Planta, 109: 61—72
Tang YS (唐玉姝), Wei CF (魏朝富), 2007. Biological indicators of soil quality progress［J］. Soils (土壤), 39 (2): 157—163
Wang FY (王凤友), 2006. The study on EeoPhysiology meehanism of seed of Cyctobalanopsis chungii［D］, 18—19
Wang HX (王贺新), Li GZ (李根柱), Yu DM (于冬梅) et al., 2008. Barrier effect of litter layer on natural regeneration of forests: A review［J］. Chinese Journal of Ecology (生态学杂志), 27 (1): 83—88
Wen H (文晖), 2010. The characteristics of seed germination and effect of light on seedling growth of Tetracentron sinense Oliv［D］. Kunming: Yunnan University (云南大学), 10—20
Wu ZY (吴征镒), Sun H (孙航), Zhou ZK (周浙昆), 2005. Origin and differentiation of endemism in the Flora of China［J］. Acta Botanica Yunnanica (云南植物研究), 27 (6): 577—604
Wu XQ (吴小巧), Huang BL (黄宝龙), Ding YL (丁雨龙), 2004. The advance on the study of protection of rare and endangered plants in China［J］. Journal of Nanjing Forestry University (Natural Sciences Edition (南京林业大学学报: 自然科学版), 28 (2): 72—76
Xiao ZS (肖治术), Wang YS (王玉山), 2003. Models of plant regeneration based on propagules of seeds［J］. Chinese Journal of Ecology (生态学杂志), 22 (4): 70—75
Xu HC (徐化成), Du YJ (杜亚娟), 1933. Leaves and seeding dynamic research of Xing An［J］. Forest Science (林业科学), 29 (4): 298—306

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