Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens

doi:10.1016/j.pld.2019.04.001

Plant Diversity ›› 2019, Vol. 41 ›› Issue (04): 266-274.DOI: 10.1016/j.pld.2019.04.001

Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens

Jianfang He^a,b, Ping Li^a,b, Heqiang Huo^c, Lina Liu^a,b, Ting Tang^a, Mingxia He^a, Junchao Huang^a, Li Liu^a

a Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming, 650201, China;
b University of Chinese Academy of Sciences, Beijing, 100049, China;
c Mid-Florida Research and Education Center, Department of Environmental Horticulture, University of Florida, FL, 32703, USA

收稿日期:2019-03-01 修回日期:2019-03-31 出版日期:2019-08-25 发布日期:2019-09-17
通讯作者: Li Liu,E-mail address:liulia@mail.kib.ac.cn
基金资助:
This work was supported by the CAS Pioneer Hundred Talents Program, the National Natural Science Foundation of China (31571262) and Yunnan Natural Science Foundation (2017FB031). We are grateful to Dr. Mitsuyasu Hasebe for providing moss spores and pPOG1 plasmid.

Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens

Jianfang He^a,b, Ping Li^a,b, Heqiang Huo^c, Lina Liu^a,b, Ting Tang^a, Mingxia He^a, Junchao Huang^a, Li Liu^a

a Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming, 650201, China;
b University of Chinese Academy of Sciences, Beijing, 100049, China;
c Mid-Florida Research and Education Center, Department of Environmental Horticulture, University of Florida, FL, 32703, USA

Received:2019-03-01 Revised:2019-03-31 Online:2019-08-25 Published:2019-09-17
Contact: Li Liu,E-mail address:liulia@mail.kib.ac.cn
Supported by:
This work was supported by the CAS Pioneer Hundred Talents Program, the National Natural Science Foundation of China (31571262) and Yunnan Natural Science Foundation (2017FB031). We are grateful to Dr. Mitsuyasu Hasebe for providing moss spores and pPOG1 plasmid.

摘要/Abstract

摘要： Heat stress can restrict plant growth, development, and crop yield. As essential plant antioxidants, carotenoids play significant roles in plant stress resistance. b-carotene hydroxylase (BHY) and b-carotene ketolase (BKT), which catalyze the conversions of b-carotene to zeaxanthin and b-carotene to canthaxanthin, respectively, are key enzymes in the carotenoid biosynthetic pathway, but little is known about their potential functions in stress resistance. Here, we investigated the roles of b-carotene hydroxylase and b-carotene ketolase during heat stress in Physcomitrella patens through expressing a b-carotene ketolase gene from Chlamydomonas reinhardtii (CrBKT) and a b-carotene hydroxylase gene from Haematococcus pluvialis (HpBHY) in the moss P. patens. In transgenic moss expressing these genes, carotenoids content increased (especially lutein content), and heat stress tolerance increased, with reduced leafy tissue necrosis. To investigate the mechanism of this heat stress resistance, we measured various physiological indicators and found a lower malondialdehyde level, higher peroxidase and superoxide dismutase activities, and higher endogenous abscisic acid and salicylate content in the transgenic plants in response to high-temperature stress. These results demonstrate that CrBKT and HpBHY increase plant heat stress resistance through the antioxidant and damage repair metabolism, which is related to abscisic acid and salicylate signaling.

关键词: Carotenoids, Heat stress, Antioxidant, Abscisic acid, Physcomitrella patens

Abstract: Heat stress can restrict plant growth, development, and crop yield. As essential plant antioxidants, carotenoids play significant roles in plant stress resistance. b-carotene hydroxylase (BHY) and b-carotene ketolase (BKT), which catalyze the conversions of b-carotene to zeaxanthin and b-carotene to canthaxanthin, respectively, are key enzymes in the carotenoid biosynthetic pathway, but little is known about their potential functions in stress resistance. Here, we investigated the roles of b-carotene hydroxylase and b-carotene ketolase during heat stress in Physcomitrella patens through expressing a b-carotene ketolase gene from Chlamydomonas reinhardtii (CrBKT) and a b-carotene hydroxylase gene from Haematococcus pluvialis (HpBHY) in the moss P. patens. In transgenic moss expressing these genes, carotenoids content increased (especially lutein content), and heat stress tolerance increased, with reduced leafy tissue necrosis. To investigate the mechanism of this heat stress resistance, we measured various physiological indicators and found a lower malondialdehyde level, higher peroxidase and superoxide dismutase activities, and higher endogenous abscisic acid and salicylate content in the transgenic plants in response to high-temperature stress. These results demonstrate that CrBKT and HpBHY increase plant heat stress resistance through the antioxidant and damage repair metabolism, which is related to abscisic acid and salicylate signaling.

Key words: Carotenoids, Heat stress, Antioxidant, Abscisic acid, Physcomitrella patens

Jianfang He, Ping Li, Heqiang Huo, Lina Liu, Ting Tang, Mingxia He, Junchao Huang, Li Liu. Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens[J]. Plant Diversity, 2019, 41(04): 266-274.

图/表 5

参考文献 44

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	可溶性有机碳 Dissolved organic carbon (g·kg^-1)	可溶性有机氮 Dissolved organic nitrogen (g·kg^-1)	紫外吸收值 Special ultraviolet visible absorption (UV)	腐殖化指标 Humification index (HIX)	分子量大小 Molecular size	pH
杉木鲜叶 Fresh leaves of Cunninghamia lanceolata	2.60 ± 0.51^a	0.005 ± 0.001^a	0.24 ± 0.01^a	0.26 ± 0.01^a	5.24 ± 0.98^a	5.98 ± 0.12^a
米槠鲜叶 Fresh leaves of Castanopsis carlesii	0.80 ± 0.11^b	0.024 ± 0.002^b	0.76 ± 0.08^b	1.75 ± 0.11^b	3.75 ± 0.10^b	5.91 ± 0.05^b
杉木凋落叶 Leaf litter of Cunninghamia lanceolata	0.99 ± 0.03^c	0.014 ± 0.001^c	1.61 ± 0.02^c	1.91 ± 0.03^c	6.90 ± 0.07^c	5.76 ± 0.05^c
米槠凋落叶 Leaf litter of Castanopsis carlesii	1.59 ± 0.02^d	0.020 ± 0.001^d	1.64 ± 0.04^c	1.90 ± 0.02^c	4.80 ± 0.30^d	4.28 ± 0.01^d

	可溶性有机碳 Dissolved organic carbon (g·kg^-1)	可溶性有机氮 Dissolved organic nitrogen (g·kg^-1)	紫外吸收值 Special ultraviolet visible absorption (UV)	腐殖化指标 Humification index (HIX)	分子量大小 Molecular size	pH
杉木鲜叶 Fresh leaves of Cunninghamia lanceolata	2.60 ± 0.51^a	0.005 ± 0.001^a	0.24 ± 0.01^a	0.26 ± 0.01^a	5.24 ± 0.98^a	5.98 ± 0.12^a
米槠鲜叶 Fresh leaves of Castanopsis carlesii	0.80 ± 0.11^b	0.024 ± 0.002^b	0.76 ± 0.08^b	1.75 ± 0.11^b	3.75 ± 0.10^b	5.91 ± 0.05^b
杉木凋落叶 Leaf litter of Cunninghamia lanceolata	0.99 ± 0.03^c	0.014 ± 0.001^c	1.61 ± 0.02^c	1.91 ± 0.03^c	6.90 ± 0.07^c	5.76 ± 0.05^c
米槠凋落叶 Leaf litter of Castanopsis carlesii	1.59 ± 0.02^d	0.020 ± 0.001^d	1.64 ± 0.04^c	1.90 ± 0.02^c	4.80 ± 0.30^d	4.28 ± 0.01^d

Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens

Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens

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