Plant Diversity ›› 2020, Vol. 42 ›› Issue (03): 174-180.DOI: 10.1016/j.pld.2020.02.001

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The leaf extract of crofton weed (Eupatorium adenophorum) inhibits primary root growth by inducing cell death in maize root border cells

Jinhu Maa, Xinxin Fengb, Xiaohuan Yangc, Yongheng Caoc, Weifeng Zhaod, Liangliang Sunc   

  1. a College of Engineering, Shanxi Agricultural University, Taigu, 030801, China;
    b College of Horticulture, Shanxi Agricultural University, Taigu, 030801, China;
    c College of Agriculture, Shanxi Agricultural University, Taigu, 030801, China;
    d Faculty of Tropical Crops, Yunnan Agricultural University, Puer, 665000, China
  • Received:2019-09-11 Revised:2020-02-12 Online:2020-06-25 Published:2020-07-15
  • Contact: Jinhu Ma
  • Supported by:
    This research was supported by the Key Project of Science and Technology of Shanxi Province (20150311016-5) and the Science and Technology innovation Foundation of Shanxi Agricultural University (2017ZZ09).

Abstract: The extract of crofton weed (Eupatorium adenophorum) inhibits seed germination and weed growth; however, the physiological mechanisms underlying the effect of crofton weed extract on the modulation of seedling growth and root system development remain largely unclear. In this study, we investigated the effects of the leaf extract of crofton weed (LECW) on primary root (PR) growth in maize seedlings. Treatment with LECW markedly inhibited seed germination and seedling growth in a dose-dependent manner. Physiological analysis indicated that the LECW induced reactive oxygen species (ROS) accumulation in root tips, thereby leading to cell swelling and deformation both in the root cap and elongation zone of root tips, finally leading to cell death in root border cells (RBCs) and PR growth inhibition. The LECW also inhibited pectin methyl esterase (PME) activity, thereby decreasing the RBC number. Taken together, our results indicated that the LECW inhibited PR growth by inducing ROS accumulation and subsequent cell death in RBCs. The present study provides a better understanding of how the LECW modifies root system development and provides insight for evaluating the toxicity of crofton weed extracts in plants.

Key words: Leaf extract of crofton weed, Maize, Cell death, Pectin methyl esterase, Root border cell