Plant Diversity ›› 2020, Vol. 42 ›› Issue (05): 323-333.DOI: 10.1016/j.pld.2020.04.003

• Articles •     Next Articles

Allelochemicals of Panax notoginseng and their effects on various plants and rhizosphere microorganisms

Yi-Jun Qiaoa,b, Cheng-Zhen Gua,c, Hong-Tao Zhua, Dong Wanga, Meng-Yue Zhangd, Yi-Xuan Zhangd, Chong-Ren Yanga, Ying-Jun Zhanga,e   

  1. a State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, China;
    b University of Chinese Academy of Sciences, Beijing, 100049, China;
    c Fujian Agriculture and Forestry University, Fuzhou, 350002, China;
    d School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, China;
    e Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China
  • Received:2019-10-07 Revised:2020-04-17 Online:2020-10-25 Published:2020-10-28
  • Contact: Dong Wang, Ying-Jun Zhang
  • Supported by:
    The authors are grateful to the members of the Analytical Group in State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, for measuring all the spectra. We also appreciate Prof. Wei-Qi Li, Dr. Yan-Xia Jia and Dr. Xing Huang for providing materials and technical support on plant bioassays. This work is supported by the Science and Technology Planning Project (2013FC008) and the Major Science and Technique Programs (2016ZF001-001) of Yunnan Province, China, and YungChi Cheng academician workstation of Yunnan provincial academy of science and technology (2015IC017).

Abstract: Panax notoginseng (Araliaceae) is an important ginseng herb with various health benefits and a history of cultivation in southwestern China over 400 years. In recent years P. notoginseng has faced serious continuous-cropping obstacles due to its large-scale cultivation. In this study, we aim to explore the allelochemicals of P. notoginseng and their interactions with various plants and rhizosphere microorganisms. The chemical constituents of the soil cultivated with 3-year-old P. notoginseng were studied by column chromatography, spectroscopic and GC-MS analyses. We identified 13 volatile components and isolated six triterpenes (1-4, 6-7) and one anthraquinone (5). Compounds 1-7 were tested for their effects on seed germination and root elongation in P. notoginseng, corn, wheat, turnip, water spinach and Arabidopsis thaliana. We also examined the effect of compounds 1-7 on the growth of ten rhizosphere microorganisms of P. notoginseng. At a concentration of 1.0 μg mL-1, compounds 3 and 5-7 caused the death of P. notoginseng root cells and compounds 2, 6 and 7 induced the death of root cells of A. thaliana. Compounds 1-5 and 7 inhibited elongation of A. thaliana root tip cells at a concentration of 10.0 μg mL-1. Moreover, at a concentration of 0.1 mg mL-1, compounds 3, 4, 6 and 7 inhibited the growth of probiotics and promoted the growth of pathogens of P. notoginseng. These results suggest that these isolated ursane-type triterpenoid acids and anthraquinone are potential allelochemicals that contribute to continuous-cropping obstacles of P. notoginseng.

Key words: Panax notoginseng, Continuous cropping obstacle, Allelochemical, Triterpenes, Anthraquinone