Functional characterization of the Arabidopsis SERRATE under salt stress

doi:10.1016/j.pld.2020.06.010

Abstract

Abstract: SERRATE (SE) plays critical roles in RNA metabolism and plant growth regulation. However, its function in stresseresponse processes remains largely unknown. Here, we examined the regulatory role of SE using the se-1 mutant and its complementation line under saline conditions. The expression of SE was repressed by salt treatment at both mRNA and protein levels. After treatment with different NaCl concentrations, the se-1 mutants showed increased sensitivity to salinity. This heightened sensitivity was evidenced by decreased germination, reduced root growth, more serious chlorosis, and increased conductivity of the mutants compared with the wild type. Further analysis revealed that SE regulates the pre-mRNA splicing of several well-characterized marker genes associated with salt stress tolerance. Our data thus imply that SE may function as a key component in plant response to salt stress by modulating the splicing of salt stress-associated genes.

Key words: SERRATE, Salt stress, Pre-mRNA alternative splicing

Minghui Mou, Qijuan Wang, Yanli Chen, Diqiu Yu, Ligang Chen. Functional characterization of the Arabidopsis SERRATE under salt stress[J]. Plant Diversity, 2021, 43(01): 71-77.

Figures/Tables 6

References 38

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尺度 Scale	MPD平均随机值 Mean MPD randomization	MPD平均实际测量值 Mean MPD analysis	标准差 SD	净相关指数 NRI	零模型 Null model
10 m×10 m	178.13	262.55	43.57	-1.97	约束型 Constrained
10 m×10 m	263.58	262.55	84.12	0.03	非约束型 Unconstrained
20 m×20 m	196.36	288.74	41.59	-2.20	约束型 Constrained
20 m×20 m	286.36	288.74	71.55	-0.01	非约束型 Unconstrained
25 m×25 m	201.98	297.13	40.38	-2.27	约束型 Constrained
25 m×25 m	293.39	297.13	68.11	-0.04	非约束型 Unconstrained

尺度 Scale	MPD平均随机值 Mean MPD randomization	MPD平均实际测量值 Mean MPD analysis	标准差 SD	净相关指数 NRI	零模型 Null model
10 m×10 m	178.13	262.55	43.57	-1.97	约束型 Constrained
10 m×10 m	263.58	262.55	84.12	0.03	非约束型 Unconstrained
20 m×20 m	196.36	288.74	41.59	-2.20	约束型 Constrained
20 m×20 m	286.36	288.74	71.55	-0.01	非约束型 Unconstrained
25 m×25 m	201.98	297.13	40.38	-2.27	约束型 Constrained
25 m×25 m	293.39	297.13	68.11	-0.04	非约束型 Unconstrained

尺度 Scale	MNND平均随机值 Mean MNND randomization	MNND平均实际测量值 Mean MNND analysis	标准差 SD	最近邻体指数 NTI	零模型 Null model
10 m×10 m	172.63	238.72	55.80	-1.110	约束型 Constrained
10 m×10 m	231.88	238.72	89.65	0.007	非约束型 Unconstrained
20 m×20 m	141.09	199.81	49.40	-1.170	约束型 Constrained
20 m×20 m	188.50	199.81	74.82	-0.024	非约束型 Unconstrained
25 m×25 m	133.57	189.81	47.05	-1.180	约束型 Constrained
25 m×25 m	179.78	189.81	69.60	-0.034	非约束型 Unconstrained

尺度 Scale	MNND平均随机值 Mean MNND randomization	MNND平均实际测量值 Mean MNND analysis	标准差 SD	最近邻体指数 NTI	零模型 Null model
10 m×10 m	172.63	238.72	55.80	-1.110	约束型 Constrained
10 m×10 m	231.88	238.72	89.65	0.007	非约束型 Unconstrained
20 m×20 m	141.09	199.81	49.40	-1.170	约束型 Constrained
20 m×20 m	188.50	199.81	74.82	-0.024	非约束型 Unconstrained
25 m×25 m	133.57	189.81	47.05	-1.180	约束型 Constrained
25 m×25 m	179.78	189.81	69.60	-0.034	非约束型 Unconstrained

尺度 Scale (m)	模型 Model	χ²	df	P
10 m×10 m	NRI vs. DBH	30.19	2	<0.0010
10 m×10 m	NTI vs. DBH	34.50	2	<0.0010
20 m×20 m	NRI vs. DBH	3.92	2	0.1409
20 m×20 m	NTI vs. DBH	14.78	2	0.0006
25 m×25 m	NRI vs. DBH	7.15	2	0.0280
25 m×25 m	NTI vs. DBH	12.64	2	0.0020
径级 DBH class
A (1 cm≤DBH< 5 cm)	NRI vs. scale	0.93	2	0.6384
A (1 cm≤DBH< 5 cm)	NTI vs. scale	6.51	2	0.0386
B (5 cm≤DBH<10 cm)	NRI vs. scale	3.69	2	0.1574
B (5 cm≤DBH<10 cm)	NTI vs. scale	0.81	2	0.6671
C (DBH≥10 cm)	NRI vs. scale	5.85	2	0.0538
C (DBH≥10 cm)	NTI vs. scale	2.61	2	0.2712