Plant Diversity ›› 2021, Vol. 43 ›› Issue (01): 86-92.DOI: 10.1016/j.pld.2020.10.003

• Articles • Previous Articles    

Diversity in seed oil content and fatty acid composition in Acer species with potential as sources of nervonic acid

Xing Hea,d, De-Zhu Lib, Bo Tiana,c   

  1. a Key Laboratory of Sustainable Utilization of Tropical Plant Resources, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 88 Xuefu Road, Kunming, Yunnan, 650223, China;
    b Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming, Yunnan, 650201, China;
    c Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Menglun, Mengla, Yunnan, 666303, China;
    d University of Chinese Academy of Sciences, Beijing, 100049, China
  • Received:2020-07-27 Revised:2020-10-21 Published:2021-03-25
  • Contact: Xing He, De-Zhu Li, Bo Tian
  • Supported by:
    We thank the Germplasm Bank of Wild Species in Southwest China and Kunming Botanical Garden, Chinese Academy of Sciences for their help in seed material collection. We thank Gong Xun, Shen Zongfang and Li Jiangying for their assistance in seed collection and the Tang Mingyong for his assistance in oil content detection. We also would like to thank the reviewers and editor for their comments and suggestions, and Editage (www.editage.com) for English language editing. This work was supported by the National Natural Science Foundation of China (grant number 31671732).

Abstract: Nervonic acid (NA, cis-15-tetracosenoic acid) is a very long-chain monounsaturated fatty acid that has been shown to be a core component of nerve fibers and nerve cells. It can be used to treat and prevent many neurological diseases. At present, commercially available NA is mainly derived from Acer truncatum seeds, which contain about 5%-6% NA in their seed oil. The aim of this study were to identify and analyze NA-containing Acer species that could be used as NA resource plants. For this purpose, 46 Acer species seeds were collected in China and in some or all of the seed oils from these species 15 fatty acids were detected, including linoleic acid, oleic acid (C18:1△9, C18:1△11), erucic acid, palmitic acid, NA, linolenic acid (C18:3△6,9,12, C18:3△9,12,15), eicosenoic acid (C20:1△11, C20:1△13), stearic acid, behenic acid, tetracosanoic acid, arachidic acid, and docosadienoic acid. Nervonic acid was detected in all samples, but the content was highly variable among species. NA content over 9% was detected in eleven species, of which Acer elegantulum had the highest levels (13.90%). The seed oil content, seed weight, and fatty acid profiles varied among species, but the comprehensive evaluation value (W) showed that A. coriaceifolium could be a new potential NA resources plant. The results also showed that NA was significantly negatively correlated with palmitic acid, oleic acid, and eicosenoic acid, but positively correlated with eicosadienoic acid, behenic acid, erucic acid, and tetracosanoic acid, which indicate the probable pathway for NA biosynthesis in Acer plants. This study has identified Acer species that may serve as NA resources and will help guide subsequent species breeding programs.

Key words: Acer, Nervonic acid, Fatty acid, Seed oil, Seed weight