Plant Diversity ›› 2022, Vol. 40 ›› Issue (01): 109-115.DOI: 10.1016/j.pld.2021.01.007

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Phytochrome B regulates jasmonic acid-mediated defense response against Botrytis cinerea in Arabidopsis

Shengyuan Xianga,b, Songguo Wua,b, Yifen Jinga,b, Ligang Chena,c, Diqiu Yua,d   

  1. a CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan, 666303, China;
    b University of Chinese Academy of Sciences, Beijing, 100049, China;
    c Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Menglun, Mengla, Yunnan, 666303, China;
    d Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, Yunnan, 666303, China
  • Received:2020-10-26 Revised:2021-01-14 Online:2022-02-25 Published:2022-03-12
  • Contact: Ligang Chen, Diqiu Yu
  • Supported by:
    We thank Prof. Rongcheng Lin (Institute of Botany, The Chinese Academy of Sciences), Hongquan Yang (Shanghai Normal University), Shengyang He (Michigan State University) for sharing research materials. We also thank Qing-Ming Qin (Jilin University) for sharing the Botrytis cinerea (B05.10) strain; Jinfeng Qi (Kunming Institute of Botany, Chinese Academy of Sciences) for his assistance in measuring JA contents and the Central Laboratory of Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences for technical supports. This work was supported by the Natural Science Foundation of China (31671275), National key R & D plan (2016YFD0101006), Yunnan Fundamental Research Projects (2019FA010).

Abstract: The phytochrome B mediated light signaling integrates with various phytohormone signalings to control plant immune response. However, it is still unclear whether phyB-mediated light signaling has an effect on the biosynthesis of jasmonate during plant defense response against Botrytis cinerea. In this study, we demonstrated that phyB-mediated light signaling has a role in this process. Initially, we confirmed that phyb plants were obviously less resistant to B. cinerea while phyB overexpressing plants showed significantly enhanced resistance. We also found that the expression of numerous JA biosynthesis genes was promoted upon treatment with red or white light when compared to that of darkness, and that this promotion is dependent on phyB. Consistent with the gene expression results, phyb plants accumulated reduced pool of JA-Ile, indicating that phyB-mediated light signaling indeed increased JA biosynthesis. Further genetic analysis showed that light-mediated JAZ9 degradation and phyB-enhanced resistance were dependent on the receptor COI1, and that pif1/3/4/5 (pifq) can largely rescue the severe symptom of phyb. Taken together, our study demonstrates that phyB may participate in plant defense against B. cinerea through the modulation of the biosynthesis of JA.

Key words: phyB, Botrytis cinerea, JA biosynthesis, COI1, JAZ, PIF