Rhododendron kuomeianum (Ericaceae), a new species from northeastern Yunnan (China), based on morphological and genomic data

doi:10.1016/j.pld.2021.04.003

Plant Diversity ›› 2021, Vol. 43 ›› Issue (04): 292-298.DOI: 10.1016/j.pld.2021.04.003

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Rhododendron kuomeianum (Ericaceae), a new species from northeastern Yunnan (China), based on morphological and genomic data

Yu-Hang Chang^a,b, Gang Yao^a,b, Jens Neilsen^c, De-Tuan Liu^a,b, Lu Zhang^d, Yong-Peng Ma^a,b

a Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China;
b Key Laboratory for Plant Diversity and Biogeography of East Asia, Chinese Academy of Sciences, Kunming, Yunnan 650201, China;
c Dobbies Garden Centre, UK-DD2 Perth, Scotland, UK;
d Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650051, China

Received:2020-09-28 Revised:2021-04-07 Online:2021-08-25 Published:2021-09-07
Contact: Yong-Peng Ma
Supported by:
We are grateful to Mrs. Rongmei Zhang for the botanical line drawing, Mr. Lianyi Li for the microscopic scanning of the leaf surface, Ms. Songting Du, Mrs. Wei Wei, and Mr. Yechun Xu for providing photos and Mr. Fengmao Yang for help during fieldwork. We also thank Mr. Shiwei Guo for his constructive comments on the manuscript. This study was financially supported by the National Natural Science Foundation of China (Grant No. 31770418 and No. 31760231) the Youth Innovation Promotion Association, Chinese Academy of Sciences (Grant No. 2018428), and the Reserve Talents for Academic and Technical Leaders of Middle-aged and Young People in Yunnan Province (Grant No. 2018HB066).

Abstract

Abstract: Rhododendron kuomeianum Y.H. Chang, J. Nielsen & Y.P. Ma, a new species of Rhododendron (Ericaceae) within subsect. Maddenia in sect. Rhododendron from Yiliang County, NE Yunnan, China, is described and illustrated. The new species is similar to R. valentinianum, but it can be easily distinguished by its sparse scales on the abaxial surface of the leaf blade, fewer flowers per inflorescence and white corolla with pale red margins. There are also differences in the widths of calyx lobes, leaf blade shape and indumentum characteristics of the petiole between the new species and Rhododendron linearilobum. We confirmed that R. kuomeianum is a new species closely related to R. valentinianum and R. changii with phylogenomic studies of 10 species within this subsection based on restriction site-associated DNA sequencing (RAD-seq) data. These phylogenomic analyses also clarified additional taxonomic problems in this subsection previously raised by morphological analysis. Our findings make a strong case for using next-generation sequencing to explore phylogenetic relationships and identify new species, especially in plants groups with complicated taxonomic problems.

Key words: New species, RAD-seq, Phylogenetic position

Yu-Hang Chang, Gang Yao, Jens Neilsen, De-Tuan Liu, Lu Zhang, Yong-Peng Ma. Rhododendron kuomeianum (Ericaceae), a new species from northeastern Yunnan (China), based on morphological and genomic data[J]. Plant Diversity, 2021, 43(04): 292-298.

References

Bradbury, P.J., Zhang, Z.W., Kroon, D.E., et al., 2007. TASSEL:software for association mapping of complex traits in diverse samples. Bioinformatics 23, 2633-2635.
Cai, L., Neilsen, J., Dao, Z.L., et al., 2016. Rhododendron longipedicellatum (Ericaceae), a new species from Southeastern Yunnan, China. Phytotaxa 282, 296-300.
Chamberlain, D.F., Hyam, R., Argent, G., et al., 1996. The genus Rhododendron:its classification and synonymy. Royal Botanic Garden Edinburgh, Edinburgh, pp. 181.
Chen, X., Huang, J.Y., Consaul, L., et al., 2010. Two new species of Rhododendron (Ericaceae) from Guizhou, China. Novon 20, 386-391.
Chen, X., Huang, J.Y., Xie, H., et al., 2012. Rhododendron cochlearifolium (Ericaceae), a new species from China. Ann. Bot. Fenn. 49, 397-402.
Danecek, P., Auton, A., Abecasis, G., et al., 2011. The variant call format and VCFtools. Bioinformatics 15, 2156-2158.
Doyle, J., 1991. DNA protocols for plants-CTAB total DNA isolation. In:Hewitt GM, Johnston A eds. Molecular techniques in taxonomy. Berlin:Springer. pp. 283-293.
Du, C., Liao S., Boufford D.E., et al., 2020, Twenty years of Chinese vascular plant novelties, 2000 through 2019. Plant Divers. 42, 393-398.
Fang, W.P., 1983. New taxa of the genus Rhododendron from China. Act. Phytotax. Sin. 21, 457-469.
Fang, R.Z., Min, T.L., 1995. The floristic study on the genus Rhododendron. Act. Bot. Yunnan. 17, 359-379.
Fang, M.Y., Fang, R.C., He, M.Y., et al.,2005. Ericaceae. In:Wu, Z.Y., Raven, P.H., Hong, D.Y. (Eds.), Flora of China, vol. 14. Science Press, Beijing & Missouri Botanical garden Press, St. louis, pp. 260-455.
Gao, L.M., Li, D.Z., 2009. Rhododendron qiaojiaense (Ericaceae), a new species from Yunnan, China. Ann. Bot. Fenn. 46, 67-70.
Gao, L.M., Zhang, S.D., 2008. Rhododendron yaoshanense (Ericaceae), a new species from NE Yunnan, China. Ann. Bot. Fenn. 45, 204-206.
Gao, L.M., Li, D.Z., Zhang, C.Q., 2003. Phylogenetic relationships of Rhododendron section Azaleastrum (Ericaceae) based on ITS sequences. Act. Phytotax. Sin. 41, 173-179.
Geng, Y.Y., 2004. New synonymies in the genus Rhododendron from China. Act. Phytotax. Sin. 42, 566-570.
Geng, Y.Y., 2014. The genus Rhododendron of China. Shanghai Scientific and Technical Publishers, Shanghai, pp. 328-347.
Gibbs, D., Chamberlain, D., Argent, G., et al., 2011. The red list of rhododendrons. Botanic Gardens Conservation International, Richmond, UK, p. 57.
Guo, C., Ma, P.F., Yang, G.Q., et al., 2020. Parallel ddRAD and genome skimming analyses reveal a radiative and reticulate evolutionary history of the temperate bamboos. https://doi.org/Syst.Biol.10.1093/sysbio/syaa076.
Heckenhauer, J., Samuel, R., Aston, P.S., et al., 2018. Phylogenomics resolves evolutionary relationships and provides insights into floral evolution in the tribe Shoreeae (Dipterocarpaceae). Mol. Phylogenet. Evol. 127, 1-13.
Hoang, D.T., Chernomor, O., Von, Haeseler. A., et al., 2018. UFBoot2:Improving the ultrafast bootstrap approximation. Mol. Biol. Evol. 2, 518-522.
Hohenlohe, P., A., Bassham, S., Etter, P., D., et al., 2010. Population genomics of parallel adaptation in threespine stickleback using sequenced RAD tags. PLoS Genet. 6, e1000862.
IUCN Standards and Petitions Committee., 2019. Guidelines for using the IUCN red list categories and criteria. Version 14. Prepared by the Standards and petitions committee of the IUCN species survival commission. http://www.iucnredlist.org/documents/RedListGuidelines.pdf.
Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K., et al., 2017. ModelFinder:fast model selection for accurate phylogenetic estimates. Nat. Methods 6, 587-589.
Li, G.Z., 1995. A revison and geographical distribution of the genus Rhododendron in Guangxi. Guihaia 15, 193-208.
Liao, R.L., Xue, D., Neilsen, J., et al., 2015. A new species of Rhododendron (Ericaceae) from Shangri-La, NW Yunnan, China. Phytotaxa 238, 293-297.
Liu, R.L., Zhong, B.T., Gao, L.M., 2018. A new species of Rhododendron (Ericaceae) from Jiangxi of China based on morphological and molecular evidences. Phytotaxa 4, 267-275.
Liu, D.T., Zhang, L., Wang, J.H., et al., 2020. Conservation genomics of a threatened Rhododendron:contrasting patterns of population structure revealed from neutral and selected SNPs. Front. Genet. 11, 757.
Ma, Y.P., Wu, Z.K., Xue, R.J., et al., 2013. A new species of Rhododendron (Ericaceae) from the Gaoligong Mountains, Yunnan, China, supported by morphological and DNA barcoding data. Phytotaxa 114, 42-50.
Ma, Y.P., Chamberlain, D.F., Sun, W.B., et al., 2015. A new species of Rhododendron (Ericaceae) from Baili Rhododendron nature reserve, NW Guizhou, China. Phytotaxa 195, 197-200.
Marczewski, T., Ma, Y.P., Zhang, X.M., et al., 2016. Why is population information crucial for taxonomy? A case study involving a hybrid swarm and related varieties. AoB Plants 8, 1-17.
Miller, M.R., Dunham, J.P., Amores, A., et al., 2007. Rapid and cost-effective polymorphism identification and genotyping using restriction site associated DNA(RAD) markers. Genome Res.2, 240-248.
Minh, B.Q., Schmidt, H.A., Chernomor, O., et al., 2020. IQ-TREE 2:New models and efficient methods for phylogenetic inference in the genomicera. Mol. Biol. Evol. 37, 1530-1534.
Price, M.N., Dehal, P.S., Arkin, A.P., 2010. FastTree 2-Approximately Maximum-Likelihood Trees for Large Alignments. PLoS One 3, e9490.
Rochette, N.C., Catchen, J.M., 2017. Deriving genotypes from Rad-seq short-read data using stacks. Nat. Protoc. 12, 2640-2659.
Rochette, N.C., Rivera-Colon, A.G., Catchen, J. M., 2019. Stacks 2:Analytical methods for paired-end sequencing improve RADseq-based population genomics. Mol. Ecol. 21, 4737-4754.
Tajima, T., 1989. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123, 585-595.
Tian, X.L., Chang, Y.H., Neilsen, J., et al., 2019. A new species of Rhododendron (Ericaceae) from northeastern Yunnan, China. Phytotaxa 395, 66-70.
Yan, L.J., Liu, J., Moller, M., et al., 2014. DNA barcoding of Rhododendron (Ericaceae), the largest Chinese plant genus in biodiversity hotspots of the Himalaya-Hengduan Mountains. Mol. Ecol. Res. 15, 932-944.
Zhang, J.L., Ma, Y.P., Wu, Z.K., et al., 2017. Natural hybridization and introgression among sympatrically distributed Rhododendron species in Guizhou, China. Biochem. Syst. Ecol. 70, 268-273.
Zhang N.N., Ma Y.P., Folk. R.A., et al., 2018. Maintenance of species boundaries in three sympatric Ligularia (Senecioneae, Asteraceae) species. J. Integer. Plant Biol. 60, 986-999.

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Rhododendron kuomeianum (Ericaceae), a new species from northeastern Yunnan (China), based on morphological and genomic data

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