BrrTCP4b interacts with BrrTTG1 to suppress the development of trichomes in Brassica rapa var. rapa

doi:10.1016/j.pld.2024.03.003

Plant Diversity ›› 2024, Vol. 46 ›› Issue (03): 416-420.DOI: 10.1016/j.pld.2024.03.003

• Short communication • Previous Articles

BrrTCP4b interacts with BrrTTG1 to suppress the development of trichomes in Brassica rapa var. rapa

Cheng Li^a,b, Li Zhang^a,b, Hefan Li^a, Yuanwen Duan^a, Xuemei Wen^c, Yongping Yang^a, Xudong Sun^a

a. The Germplasm Bank of Wild Species, Yunnan Key Laboratory of Crop Wild Relatives Omics, Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
b. University of Chinese Academy of Sciences, Beijing, China;
c. Tibet Plateau Institute of Biology, Lhasa 850001, Tibet, China

Received:2023-10-19 Revised:2024-03-01 Published:2024-05-20
Contact: Yongping Yang,E-mail:yangyp@mail.kib.ac.cn;Xudong Sun,E-mail:sunxudong@mail.kib.ac.cn
Supported by:
This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences, Pan-Third Pole Environment Study for a Green Silk Road (Pan-TPE) (XDA2004010306) and the Second Tibetan Plateau Scientific Expedition and Research (STEP) program (2019QZKK0502), and Science and Technology Program of Xizang Autonomous Region (XZ202001ZY0003G).

Abstract

Abstract: The number of trichomes significantly increased in CRISPR/Cas9-edited BrrTCP4b turnip (Brassica rapa var. rapa) plants. However, the underlying molecular mechanism remains to be uncovered. In this study, we performed the Y2H screen using BrrTCP4b as the bait, which unveiled an interaction between BrrTCP4b and BrrTTG1, a pivotal WD40-repeat protein transcription factor in the MYB-bHLH-WD40 (MBW) complex. This physical interaction was further validated through bimolecular luciferase complementation and co-immunoprecipitation. Furthermore, it was found that the interaction between BrrTCP4b and BrrTTG1 could inhibit the activity of MBW complex, resulting in decreased expression of BrrGL2, a positive regulator of trichomes development. In contrast, AtTCP4 is known to regulate trichomes development by interacting with AtGL3 in Arabidopsis thaliana. Overall, this study revealed that BrrTCP4b is involved in trichome development by interacting with BrrTTG1 in turnip, indicating a divergence from the mechanisms observed in model plant A. thaliana. The findings contribute to our understanding of the regulatory mechanisms governing trichome development in the non-model plants turnip.

Key words: TCP transcription factor, MBW complex, Trichome development, Turnip

Cheng Li, Li Zhang, Hefan Li, Yuanwen Duan, Xuemei Wen, Yongping Yang, Xudong Sun. BrrTCP4b interacts with BrrTTG1 to suppress the development of trichomes in Brassica rapa var. rapa[J]. Plant Diversity, 2024, 46(03): 416-420.

References

[1] Airoldi, C.A., Hearn, T.J., Brockington, S.F., et al., 2019. TTG1 proteins regulate circadian activity as well as epidermal cell fate and pigmentation. Nat Plants 5(11):1145-1153.
[2] Birchler, J.A., Yang, H., 2022. The multiple fates of gene duplications:Deletion, hypofunctionalization, subfunctionalization, neofunctionalization, dosage balance constraints, and neutral variation. Plant Cell 34(7):2466-2474.
[3] Cheng, F., Wu, J., Wang, X., 2014. Genome triplication drove the diversification of Brassica plants. Horticulture Research 1(1):14024.
[4] Hauser, M.T., 2014. Molecular basis of natural variation and environmental control of trichome patterning. Front Plant Sci 5320.
[5] Ishida, T., Kurata, T., Okada, K., et al., 2008. A genetic regulatory network in the development of trichomes and root hairs. Annu Rev Plant Biol 59365-386.
[6] Lan, J., Zhang, J., Yuan, R., et al., 2021. TCP Transcription Factors Suppress Cotyledon Trichomes by Impeding a Cell Differentiation-regulating Complex. Plant Physiol 186434-451.
[7] Liu, Y., Zhang, L., Li, C., et al., 2022. Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa. Plant Methods 18(1):98.
[8] Meng, L.S., Li, C., Xu, M.K., et al., 2018. Arabidopsis ANGUSTIFOLIA3(AN3) is associated with the promoter of CONSTITUTIVE PHOTOMORPHOGENIC1(COP1) to regulate light-mediated stomatal development. Plant, Cell& Environment 41(7):1645-1656.
[9] Oppenheimer, D.G., Herman, P.L., Sivakumaran, S., et al., 1991. A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules. Cell 1(67):483-493.
[10] Payne, C.T., Zhang, F., Lloyd, A.M., 2000. GL3 encodes a bHLH protein that regulates trichome development inI through interaction with GL1 and TTG1. Genetics 156(3):1349-1362.
[11] Rerie, W.G., Feldmann, K.A., Marks, M.D., 1994. The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis. Genes Dev 8(12):1388-1399.
[12] Walker, A.R., Davison, P.A., Bolognesi-Winfield, A.C., et al., 1999. The TRANSPARENT TESTA GLABRA1 locus, which regulates trichome differentiation and anthocyanin biosynthesis in Arabidopsis, encodes a WD40 repeat protein. Plant Cell 11(7):1337-1350.
[13] Wang, X., Shen, C., Meng, P., et al., 2021. Analysis and review of trichomes in plants. BMC Plant Biol 21(1):70.
[14] Zhao, M., Morohashi, K., Hatlestad, G., et al., 2008. The TTG1-bHLH-MYB complex controls trichome cell fate and patterning through direct targeting of regulatory loci. Development 135(11):1991-1999.

BrrTCP4b interacts with BrrTTG1 to suppress the development of trichomes in Brassica rapa var. rapa

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 6

Recommended Articles

Metrics

[1]	Boqun Li, Di Chen, Yongping Yang, Xiong Li. Effects of soil properties on accumulation characteristics of copper, manganese, zinc, and cadmium in Chinese turnip [J]. Plant Diversity, 2019, 41(05): 340-346.
[2]	Yuansheng Wu, Xiong Li, Di Chen, Xi Han, Boqun Li, Yonghong Yang, Yongping Yang. Comparative expression analysis of heavy metal ATPase subfamily genes between Cd-tolerant and Cd-sensitive turnip landraces [J]. Plant Diversity, 2019, 41(04): 275-283.
[3]	Yanan Pu, Danni Yang, Xin Yin, Qiuli Wang, Qian Chen, Yunqiang Yang, Yongping Yang. Genome-wide analysis indicates diverse physiological roles of the turnip (Brassica rapa var. rapa) oligopeptide transporters gene family [J]. Plant Diversity, 2018, 40(02): 57-67.
[4]	Yan Zheng, Landi Luo, Yuanyuan Liu, Yunqiang Yang, Chuntao Wang, Xiangxiang Kong, Yongping Yang. Effect of vernalization on tuberization and flowering in the Tibetan turnip is associated with changes in the expression of FLC homologues [J]. Plant Diversity, 2018, 40(02): 50-56.
[5]	Xiong Li, Xiaoming Zhang, Yuansheng Wu, Boqun Li, Yongping Yang. Physiological and biochemical analysis of mechanisms underlying cadmium tolerance and accumulation in turnip [J]. Plant Diversity, 2018, 40(01): 19-27.
[6]	Yuanyuan Liu, Xin Yin, Ya Yang, Chuntao Wang, Yongping Yang. Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family [J]. Plant Diversity, 2017, 39(03): 123-129.