Plant Diversity ›› 2008, Vol. 30 ›› Issue (04): 457-463.DOI: 10.3724 SP.J.1143.2008.07228
• Articles •
The plant expression vector containing human alpha-lactalbumin gene was constructed and its gene was transformed into tobacco using leaf disc mediated by Agrobacterium tumefaciens LBA 4404 as a vehicle. PCR and Southern blot analyses showed that human alpha-lactalbumin gene was integrated into the tobacco genome. RT-PCR and histochemical staining for GUS activity demonstrated that human alpha-lactalbumin gene was expressed in transgenic tobacco plants. Further analysis of the data indicated that the average of cysteine contents in 9 transgenic tobacco plants was greatly increased by 166.40% besides one of them which was increased by 318.02%
Human alpha-lactalbumin gene
WANG Yi-Qun. Transformation of Human Alpha-lactalbumin Gene into Tobacco and Improvement of Cysteine Contents in Transgenic Tobacco Plants[J]. Plant Diversity, 2008, 30(04): 457-463.
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