Plant Diversity ›› 2008, Vol. 30 ›› Issue (04): 457-463.DOI: 10.3724 SP.J.1143.2008.07228

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Transformation of Human Alpha-lactalbumin Gene into Tobacco and Improvement of Cysteine Contents in Transgenic Tobacco Plants

WANG Yi-Qun   

  1. Laboratory of Developmental and Neural Biology , College of Life Sciences, Fujian Normal University , Fuzhou 350108 , China
  • Received:2007-10-08 Online:2008-08-25 Published:2008-08-25
  • Contact: WANG Yi-Qun

Abstract: The plant expression vector containing human alpha-lactalbumin gene was constructed and its gene was transformed into tobacco using leaf disc mediated by Agrobacterium tumefaciens LBA 4404 as a vehicle. PCR and Southern blot analyses showed that human alpha-lactalbumin gene was integrated into the tobacco genome. RT-PCR and histochemical staining for GUS activity demonstrated that human alpha-lactalbumin gene was expressed in transgenic tobacco plants. Further analysis of the data indicated that the average of cysteine contents in 9 transgenic tobacco plants was greatly increased by 166.40% besides one of them which was increased by 318.02%

Key words: Human alpha-lactalbumin gene

CLC Number: