Plant Diversity ›› 2008, Vol. 30 ›› Issue (6): 706-712.DOI: 10.3724 SP.J.1143.2008.08037

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Cloning and Sequencing for ISSR Specific Fragments in Cytoplasmic Male Sterile, Maintainer and Restorer Lines of Ramie ( Boehmeria nivea)

DUAN Ji-Qiang1 , 2 , LI Jian-Yong1 , WU Li-Yan1 , LIANG Xue-Ni1 , LIU Fei-Hu1   

  1. 1 Laboratory of Plant Improvement and Utilization , Yunnan University , Kunming 650091 , China ;
    2 Nujiang Food and Drug Administration , Nujiang 673100 , China
  • Received:2008-03-13 Online:2008-12-25 Published:2008-12-25
  • Contact: LIU Fei-Hu

Abstract: The polymorphic analysis of mtDNA from ramie cytoplasmic male sterile (MS) , maintainer (M) and restorer (R) lines ( three lines) were performed by using ISSR technique . Thirty-eight ISSR primers were used for testing mtDNA
polymorphism and different amplified bands were observed in six primers in ramie“three lines”, these specific bands were cloned and sequenced . Among those bands, the fragment 21-MS (GenBank accession number : EU122345) had 956 bp in total length and a 525 bp intact coding region that encoded a polypeptide consisting of 174 amino acids . Fragment 31-M􊄯R
(GenBank accession number : EU122344) had 778 bp in total length and a 404 bp incompletely coding region that encoded a polypeptide consisting of 134 amino acids; this fragment had 71%- 76% and 73%- 77% of homology in nucleotide and amino acid sequences with lycopene beta-cyclase allozyme gene in many other plants .

Key words: Ramie ( Boehmeria nivea)

CLC Number: