Abstract: The polymorphic analysis of mtDNA from ramie cytoplasmic male sterile (MS) , maintainer (M) and restorer (R) lines ( three lines) were performed by using ISSR technique . Thirty-eight ISSR primers were used for testing mtDNA
polymorphism and different amplified bands were observed in six primers in ramie“three lines”, these specific bands were cloned and sequenced . Among those bands, the fragment 21-MS (GenBank accession number : EU122345) had 956 bp in total length and a 525 bp intact coding region that encoded a polypeptide consisting of 174 amino acids . Fragment 31-M􊄯R
(GenBank accession number : EU122344) had 778 bp in total length and a 404 bp incompletely coding region that encoded a polypeptide consisting of 134 amino acids; this fragment had 71%- 76% and 73%- 77% of homology in nucleotide and amino acid sequences with lycopene beta-cyclase allozyme gene in many other plants .

Key words: Ramie ( Boehmeria nivea)