SNP Analysis of Six Nuclear Gene Fragments for Studying Population Genetics of Oryza granulata from China

doi:10.7677/ynzwyj201312151

Abstract

Abstract:

A total of six rice genes of functional importance, including Osgstf3, OsNAC6, BADH, XCP2, EMF2 and ERCC4, were selected to assess suitable SNPs for use in an analysis of population genetic structure of Oryza granulata, a precious wild relative of Asian cultivated rice. An initial survey of 12 representative samples of O.granulata from Yunnan and Hainan, China, resolved few SNPs in Osgstf3, OsNAC6, BADH and XCP2, while low levels of variation were recorded within introns of EMF2 and ERCC4. A subsequent SNP survey was conducted within the EMF2 and ERCC4 fragments across a sample of 52 plants collected from three and two natural populations in Yunnan and Hainan, respectively. This survey showed that O.granulata exhibits fairly high genetic divergence between populations despite low levels of nucleotide diversity. Genetic divergence between populations was evident both between and within geographical regions. These findings imply that recent habitat fragmentation in tropical and subtropical forests led to severe restrictions to gene flow, which enhanced divergence among populations.

Key words: Nuclear gene, Oryza granulata, Single nucleotide polymorphisms, Genetic variation, Population genetic structure

CLC Number:

Q 751

WU Chun-Yan-, GAO Li-Zhi. SNP Analysis of Six Nuclear Gene Fragments for Studying Population Genetics of Oryza granulata from China[J]. Plant Diversity, 2013, 35(5): 537-546.

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