Plant Diversity ›› 2014, Vol. 36 ›› Issue (03): 375-380.doi: 10.7677/ynzwyj201413156

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A Modified Protocol for Plant Genome DNA Extraction

 CHEN  Lin-Yang-1**, SONG  Min-Shu-2, ZHA  Hong-Guang-2, LI  Zhi-Min-1   

  1. 1 School of Life Science, Yunnan Normal University, Kunming 650092, China; 2 Key Laboratory for Plant Diversity and
    Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2013-07-25 Online:2014-05-25 Published:2013-12-20
  • Supported by:

    国家基金委-云南省联合基金重点项目 (U1B6601)、国家自然科学基金 (30360049)、国家自然科学基金 (31170216)、云南省教学名师工程项目

Abstract:

 Extracting high quality genome DNA from plant is a vital step in plant molecular biology research. But it is relatively difficult to obtain high quality DNA from plant species which are rich in polysaccharides, polyphenols, and other secondary metabolites. This method was developed bases on common CTAB and commercial kit method with some modifications and features removing interfering material prior to the cell lysis. High quality DNA was obtained by this method from a variety of plant species and evaluated by electrophoresis and spectrometer methods. We demonstrated that it is a common plant genomic DNA extraction method especially suitable for some difficult plant species.

Key words: Plant genome DNA, Modified common DNA extraction method, Plant material

CLC Number: 

  • Q 78, Q 943