Abstract: Crown gall tissue culture lines fo Lithospermum erythizon were estadilshed dy transformation of steile seedlings weth Agrobacterium tumefaciens C58.Nopaline was inspected in the crown gall snd showed that the transformation was successfull.L6 line, can secret red naphthoquinone pigments that are shikonin derivatives on phytohormone-free 6,7-V medium was selected from other lines on different basic phyou-hormone-free media (MS,6,7-V,WP,B5).The content of shikonin in the ceown gall was 1,18% (dry tissue).The study showed that crown gall tissue culture of L.erythrorhizon was a new method to pro-duce shikonin. 　

Key words: Lithospermum erythrohizon