Karyotypes of Twenty-five Populations of Thirteen Species in Nomocharis and Lilium

doi:10.3724/SP.J.1143.2011.11017

Abstract

Abstract:

Karyotypes of 11 populations of 6 species in Nomocharis and 14 populations of 7 species in Lilium were analyzed to discuss the taxonomic treatment of Nomocharis and elucidate the phylogenetic relationship between Nomocharis and Lilium. Cytological observations were carried out exclusively from root tips. For mitotic arrest, meristematic tissues were pre-treated with 1∶1 mixture of 0.1% colchicine and p-dichlorobenzene at ambient temperature (10℃-20℃) for 8-10h before fixation in Carnoy I (one part glacial acetic acid to three parts absolute ethanol) at 4℃ for 2-24h. They were macerated in 1mol·L-1 HCl at 60℃ for 5-6 minutes, stained and squashed in Carbol Fuchisn. Karyotype asymmetry was assessed by four indices (As.K%, AI, CVCL and CVCI) in which the later three were newly proposed and had be considered to have phylogenetic significance. Furthermore, we tried to clarify the relationship between Nomocharis and Lilium with the scatter diagram of CVCL against CVCI. The results and conclusions were as follows：1) Karyotypes showed differences among species and populations, especially in the number and location of secondary constrictions as well as the presence or absence of B chromosomes；2) Nomocharis and Lilium are closely related；3) Chromosome structural variation produced abundant types (five in total) of secondary constrictions, which was considered to be the main driving force of evolution in the genus Nomocharis；4) Aneuploid, triploid, B chromosomes and secondary constriction type Ⅲ emerged accompanied by the dispersal of Nomocharis to adjacent areas from its origin and differentiation center, namely, Gaoligongshan. In this paper, the triploid of N.basilissa and karyotypes of L.xanthellum were documented for the first time.

Key words: Karyotype evolution, Nomocharis, Lilium, Secondary constriction, The AI index

CLC Number:

Q 942

Wan Juan, ZHOU Song-Dong. Karyotypes of Twenty-five Populations of Thirteen Species in Nomocharis and Lilium[J]. Plant Diversity, 2011, 33(5): 477-.

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