Protein Level Analysis of Kobresia pygmaea (Cyperaceae) Response to Diurnal Environment on the Tibetan Plateau

doi:10.7677/ynzwyj201514070

Abstract

Abstract:

Kobresia pygmaea is an important constructive species of alpine meadow on the Tibetan Plateau; its growth and development is influenced by both drastic environments of seasonal or interannual replacement and diurnal cycle. For a long time, studies about Kpygmaea adaption to alpine environment were mainly focused on the longterm adaptation while the diurnal responses were rarely reported. In the present study, we performed comparative proteomics approach, together with antioxidant enzyme assays and western blot, to analyze the variation of proteins expression in Kpygmaea every four hours from 2 am. to 22 pm. in a day, which were collected from elevation of 4800m on the Nyainqentanglha Mountains. The results implicated that Kpygmaea was subjected to timeperiod abiotic environmental stresses, including high temperature, intense light and ultraviolet radiation in the day and low temperature in the night. To maintain normal life activities, Kpygmaea formed a complex set of strategies to deal with the potential damage. These strategies at least contained the plasticity and flexibility of antioxidant system, heat shock proteins accumulation, and abscisic acid metabolism. Meanwhile, a potential way named timespecial activities regulated by proteins was also used to improve efficiency of survival, which meant some important biological processes, such as energy metabolism and photosynthesis, mostly occurred at more suitable time to avoid disadvantageous periods. These results supplied more knowledge about alpine plants adaptation to extreme day and night on the Tibetan Plateau.

Key words: Kobresia pygmaea, Tibetan Plateau, Diurnal environment, Proteomics, Antioxidant enzyme

CLC Number:

Q 945.79

LI Xiong, YANG Yun-Qiang, YANG Shi-Hai, MA Lan, KONG Xiang-Xiang, HU Xiang-Yang, YANG Yong-Ping. Protein Level Analysis of Kobresia pygmaea (Cyperaceae) Response to Diurnal Environment on the Tibetan Plateau[J]. Plant Diversity, 2015, 37(2): 145-156.

References

Alabadi D, Oyama T, Yanovsky MJ et al., 2001. Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis diurnal clock［J］. Science, 293: 880—883
Annicchiarico P, Bozzo F, Parente G et al., 1995. Analysis of adaptation of grass/legume mixtures to Italian alpine and subalpine zones through an additive main effects and multiplicative interaction model［J］. Grass and Forage Science, 50: 405—413
Apel K, Hirt H, 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction［J］. Annual Review of Plant Biology, 55: 373—399
Bai XG, Yang LM, Yang YQ et al., 2011. Deciphering the protective role of nitric oxide against salt stress at the physiological and proteomic levels in maize［J］. Journal of Proteome Research, 10: 4349—4364
Barbosa H, Slater NKH, Marcos JC, 2009. Protein quantification in the presence of poly (ethylene glycol) and dextran using the Bradford method［J］. Analytical Biochemistry, 395: 108—110
Beaucham C, Fridovic I, 1971. Superoxide dismutaseimproved assays and an assay applicable to acrylamide gels［J］. Analytical Biochemistry, 44: 276—287
Damerval C, Devienne D, Zivy M et al., 1986. Technical improvements in twodimensional electrophoresis increase the level of geneticvariation detected in wheatseedling proteins［J］. Electrophoresis, 7: 52—54
Doyle MR, Davis SJ, Bastow RM et al., 2002. The ELF4 gene controls diurnal rhythms and flowering time in Arabidopsis thaliana［J］. Nature, 419: 74—77
Farre EM, Harmer SL, Harmon FG et al., 2005. Overlapping and distinct roles of PRR7 and PRR9 in the Arabidopsis diurnal clock［J］. Current Biology, 15: 47—54
Fujita M, Fujita Y, Noutoshi Y et al., 2006. Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks［J］. Current Opinion in Plant Biology, 9: 436—442
Kato S, Yamagishi K, Tatsuzawa F et al., 1993. Identification of cytoplasmic and nuclear lowmolecularweight heatshock proteins in tomato fruit［J］. Plant and Cell Physiology, 34: 367—370
Kosova K, Vitamvas P, Prasil IT, 2011. Expression of dehydrins in wheat and barley under different temperatures［J］. Plant Science, 180: 46—52
Laemmli UK, Beguin F, Gujerkel G, 1970. A factor preventing major head protein of bacteriophage T4 from random aggregation［J］. Journal of Molecular Biology, 47: 69—74
Lai AG, Doherty CJ, MuellerRoeber B et al., 2012. DIURNAL CLOCKASSOCIATED 1 regulates ROS homeostasis and oxidative stress responses［J］. Proceedings of the National Academy of Sciences of the United States of America, 109: 17129—17134
Lee DH, Lee CB, 2000. Chilling stressinduced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays［J］. Plant Science, 159: 75—85
Li X, Hu XY, Yang YP, 2013. Research of important forage Kobresia pygmaea in QinghaiTibet Plateau［J］. Pruataculture & Animal Husbandry, 1: 30—39
Liu YF, Qi MF, Li TL, 2012. Photosynthesis, photoinhibition, and antioxidant system in tomato leaves stressed by low night temperature and their subsequent recovery［J］. Plant Science, 196: 8—17
Lou B, Xu DD, Xu HX et al., 2011. Effect of high water temperature on growth, survival and antioxidant enzyme activities in the Japanese flounder Paralichthys olivaceus［J］. African Journal of Agricultural Research, 6: 2875—2882
Lud D, Moerdijk TCW, Van de Poll WH et al., 2002. DNA damage and photosynthesis in Antarctic and Arctic Sanionia uncinata (Hedw.) Loeske under ambient and enhanced levels of UVB radiation［J］. Plant Cell and Environment, 25: 1579—1589
Miehe G, Mlehe S, Kaiser K et al., 2008. Status and dynamics of Kobresia pygmaea ecosystem on the Tibetan plateau［J］. Ambio, 37: 272—279
Mittler R, 2002. Oxidative stress, antioxidants and stress tolerance［J］. Trends in Plant Science, 7: 405—410
Nakano Y, AsAda K, 1981. Hydrogenperoxide is scavenged by ascorbatespecific peroxidase in spinachchloroplasts［J］. Plant and Cell Physiology, 22: 867—880
Oquist G, Hurry VM, Huner NPA, 1993. Lowtemperature effects on Photosynthesis and correlation with freezing tolerance in spring and winter cultivars of wheat and rye［J］. Plant Physiology, 101: 245—250
Poulson ME, Boeger MRT, Donahue RA, 2006. Response of photosynthesis to high light and drought for Arabidopsis thaliana grown under a UVB enhanced light regime［J］. Photosynthesis Research, 90: 79—90
PrunedaPaz JL, Kay SA, 2010. An expanding universe of diurnal networks in higher plants［J］. Trends in Plant Science, 15: 259—265
Siaussat D, Laparie M, Maria A et al., 2013. Heat shock protein responses to salinity, food deprivation, and temperature in the invasive ground beetle Merizodus soledadinus at the Kerguelen Islands［J］. Polar Biology, 36: 201—209
Somers DE, 1999. The physiology and molecular bases of the plant diurnal clock［J］. Plant Physiology, 121: 9—19
Varga B, Janda T, Laszlo E et al., 2012. Influence of abiotic stresses on the antioxidant enzyme activity of cereals［J］. Acta Physiologiae Plantarum, 34: 849—858
Wang Y, Meng LL, Yang YP et al., 2010. Change in floral orientation in Anisodus luridus (Solanaceae) protects pollen grains and facilitates development of fertilized ovules［J］. American Journal of Botany, 97: 1618—1624
Wang ZY, Tobin EM, 1998. Constitutive expression of the DIURNAL CLOCK ASSOCIATED 1 (CCA1) gene disrupts diurnal rhythms and suppresses its own expression［J］. Cell, 93: 1207—1217
Yang Y, Wang GX, Klanderud K et al., 2011. Responses in leaf functional traits and resource allocation of a dominant alpine sedge (Kobresia pygmaea) to climate warming in the QinghaiTibetan Plateau permafrost region［J］. Plant and Soil, 349: 377—387
Yang Y, Wang GX, Yang LD et al., 2012. Physiological responses of Kobresia pygmaea to warming in QinghaiTibetan Plateau permafrost region［J］. Acta OecologicaInternational Journal of Ecology, 39: 109—116
Yu C, Huang SJ, Hu XM et al., 2013. Changes in photosynthesis, chlorophyll fluorescence, and antioxidant enzymes of mulberry (Morus ssp.) in response to salinity and hightemperature stress［J］. Biologia, 68: 404—413Zhang LY, Turkington R, Tang Y, 2010. Flowering and fruiting phenology of 24 plant species on the north slope of Mt. Qomolangma (Mt. Everest) ［J］. Journal of Mountain Science, 7: 45—54Zhou HH, Chen YN, Li WH et al., 2010. Photosynthesis of Populus euphratica in relation to groundwater depths and high temperature in arid environment, northwest China［J］. Photosynthetica, 48: 257—268

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