Characterization of an algal phosphomannose isomerase gene and its application as a selectable marker for genetic manipulation of tomato

doi:10.1016/j.pld.2020.06.001

Abstract

Abstract: Establishing a transgenic plant largely relies on a selectable marker gene that can confer antibiotic or herbicide resistance to plant cells. The existence of such selectable marker genes in genetically modified foods has long been criticized. Plant cells generally exhibit too low an activity of phosphomannose isomerase (PMI) to grow with mannose as a sole carbon source. In this study, we characterized PMI from the green microalga Chlorococcum sp. and assessed its feasibility as a selectable marker for plant biotechnology. Chlorococcum sp. PMI (ChlPMI) was shown to be closely related to higher plants but more distant to bacterial counterparts. Overexpression of ChlPMI in tomato induced callus and shoot formation in media containing mannose (6 g/L) and had an average transformation rate of 3.9%. Based on this transformation system, a polycistronic gene cluster containing crtB, HpBHY, CrBKT and SlLCYB (BBBB) was co-expressed in a different tomato cultivar. Six putative transformants were achieved with a transformation rate of 1.4%, which produced significant amounts of astaxanthin due to the expression of the BBBB genes. Taken together, these findings indicate that we have established an additional tool for plant biotechnology that may be suitable for genetically modifying foods safely.

Key words: Algae, Tomato, Transformation, Phosphomannose isomerase, Astaxanthin

Yuanyuan Lin, Junchao Huang. Characterization of an algal phosphomannose isomerase gene and its application as a selectable marker for genetic manipulation of tomato[J]. Plant Diversity, 2021, 43(01): 63-70.

Figures/Tables 9

References 66

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年份 Year	总物种 Total species	本土植物 Native species	非本土植物 Non-native species	广布物种 Widespread species
2011	33.50 ± 5.04^a	30.00 ± 4.71^a	3.50 ± 0.50^a	23.50 ± 3.57^a
2012	35.50 ± 8.50^a	31.50 ± 8.06^a	4.00 ± 1.08^a	29.00 ± 4.65^a
2013	43.25 ± 6.92^a	39.75 ± 5.43^a	3.50 ± 1.65^a	19.75 ± 3.49^a

年份 Year	总物种 Total species	本土植物 Native species	非本土植物 Non-native species	广布物种 Widespread species
2011	33.50 ± 5.04^a	30.00 ± 4.71^a	3.50 ± 0.50^a	23.50 ± 3.57^a
2012	35.50 ± 8.50^a	31.50 ± 8.06^a	4.00 ± 1.08^a	29.00 ± 4.65^a
2013	43.25 ± 6.92^a	39.75 ± 5.43^a	3.50 ± 1.65^a	19.75 ± 3.49^a

年份 Year	Shannon-Wiener指数 Shannon-Wiener index	均匀度指数 Pielou index	优势度指数 Simpson index
2011	3.255 ± 0.202^a	0.936 ± 0.016^a	0.946 ± 0.029^a
2012	3.033 ± 0.359^a	0.875 ± 0.037^a	0.902 ± 0.079^a
2013	3.551 ± 0.195^a	0.954 ± 0.008^a	0.964 ± 0.015^a

年份 Year	Shannon-Wiener指数 Shannon-Wiener index	均匀度指数 Pielou index	优势度指数 Simpson index
2011	3.255 ± 0.202^a	0.936 ± 0.016^a	0.946 ± 0.029^a
2012	3.033 ± 0.359^a	0.875 ± 0.037^a	0.902 ± 0.079^a
2013	3.551 ± 0.195^a	0.954 ± 0.008^a	0.964 ± 0.015^a

科名 Family name	中文名 Chinese name	拉丁名 Latin name	作者赋值CC value by authors	专家赋值平均值 Mean CC value by experts	专家赋值标准差 Standard deviation of mean CC value by experts	最终值 Final CC value
卷柏科Selaginellaceae	伏地卷柏	Selaginella nipponica	8	7.5	0.500	8
	翠云草	Selaginella uncinata	8	7.8	0.433	8
水韭科 Isoetaceae 木贼科 Equisetaceae	中华水韭	Isoetes sinensis	10	9.8	0.433	10
	犬问荆	Equisetum palustre	4	3.5	0.500	4
	问荆	Equisetum arvense	1	1.0	0.000	1
	溪木贼	Equisetum fluviatile	4	4.3	0.829	4
	木贼	Equisetum hyemale	1	1.0	0.000	1
	披散木贼	Equisetum diffusum	1	1.0	0.000	1
	笔管草	Equisetum ramosissimum subsp. debile	1	1.0	0.000	1
	节节草	Equisetum ramosissimum	1	1.0	0.000	1
紫箕科 Osmundaceae	华南紫萁	Osmunda vachellii	3	2.3	0.433	3
	紫箕	Osmunda japonica	2	1.3	0.433	2
海金沙科 Lygodiaceae	海金沙	Lygodium japonicum	1	1.0	0.000	1
蚌壳蕨科 Dicksoniaceae	金毛狗	Cibotium barometz	6	6.0	0.707	6
骨碎补科Oleandracea	肾厥	Nephrolepis auriculata	7	7.3	0.433	7
铁线蕨科 Adiantaceae	铁线蕨	Adiantum capillus-veneris	2	1.5	0.500	2
	扇叶铁线蕨	Adiantum flabellulatum	8	8.0	0.707	8
里白科 Gleicheniaceae	里白	Hicriopteris glauca	5	4.8	0.829	5
	光里白	Hicriopteris laevissima	6	5.5	0.500	6
姬蕨科 Dennstaedtiaceae	边缘鳞盖蕨	Microlepia marginata	8	7.8	0.433	8
	姬蕨	Hypolepis punctata	7	7.3	0.433	7
水蕨科 Parkeriaceae	水蕨	Ceratopteris thalictroides	10	9.8	0.433	10
蹄盖蕨科 Athyriaceae	峨眉介蕨	Dryoathyrium unifurcatum	10	9.8	0.433	10
	假蹄盖蕨	Athyriopsis japonica	7	6.5	1.118	7
	尖头蹄盖蕨	Athyrium vidalii	7	7.0	0.707	7
	菜蕨	Callipteris esculenta	6	6.3	0.433	6
中国蕨科Sinopteridaceae	毛轴碎米蕨	Cheilosoria chusana	6	6.3	0.433	6
金星蕨科 Thelyptheridaceae	渐尖毛蕨	Cyclosorus acuminatus	7	7.0	0.707	7
	延羽卵果蕨	Phegopteris decursivepinnata	5	5.3	0.433	5
	沼泽蕨	Thelypteris palustris	5	5.3	0.829	5
	金星蕨	Parathelypteris glanduligera	1	1.0	0.000	1
	紫柄蕨	Pseudophegopteris pyrrhorachis	8	8.3	0.433	8
凤毛蕨科Pteridaceae	井栏边草	Pteris multifida	4	4.3	0.829	4
	蜈蚣蕨	Pteris vittata	1	1.0	0.000	1
蕨科Pteridiaceae	蕨	Pteridium aquilinum subsp. latiusculum	4	3.8	0.707	4
鳞毛蕨科 Dryopyeridaceae	稀羽鳞毛蕨	Dryopteris sparsa	1	1.0	0.000	1
	倒鳞鳞毛蕨	Dryopteris reflexosquamata	8	7.5	0.707	8
乌毛蕨科 Blechnaceae	胎生狗脊蕨	Woodwardia prolifera	7	7.0	0.707	7
	乌毛蕨	Blechnum orientale	9	9.2	0.707	9
球子蕨科 Onocleaceae	中华荚果蕨	Matteuccia intermedia	7	7.3	0.829	7
	荚果蕨(黄果香)	Matteuccia struthiopteris	7	7.3	1.090	7
满江红科 Azollaceae	满江红	Azolla pinnata subsp. asiatica	1	1	0.000	1
苹科 Marsileaceae	苹	Marsilea quadrisolia	1	1.0	0.000	1
槐叶苹科 Salviniaceae	槐叶苹	Salvinia natans	1	1.0	0.000	1