Accession-specific flowering time variation in response to nitrate fluctuation in Arabidopsis thaliana

doi:10.1016/j.pld.2020.05.004

Abstract

Abstract: Flowering time, a key transition point from vegetative to reproductive growth, is regulated by an intrinsic complex of endogenous and exogenous signals including nutrient status. For hundreds of years, nitrogen has been well known to modulate flowering time, but the molecular genetic basis on how plants adapt to ever-changing nitrogen availability remains not fully explored. Here we explore how Arabidopsis natural variation in flowering time responds to nitrate fluctuation. Upon nitrate availability change, we detect accession- and photoperiod-specific flowering responses, which also feature a accession-specific dependency on growth traits. The flowering time variation correlates well with the expression of floral integrators, SOC1 and FT, in an accession-specific manner. We find that gene expression variation of key hub genes in the photoperiod-circadian-clock (GI), aging (SPLs) and autonomous (FLC) pathways associates with the expression change of these integrators, hence flowering time variation. Our results thus shed light on the molecular genetic mechanisms on regulation of accession- and photoperiod-specific flowering time variation in response to nitrate availability.

Key words: Nitrate, Arabidopsis thaliana, Photoperiod, Natural variation, Flowering time, Gene expression

Fei-Hong Yan, Li-Ping Zhang, Fang Cheng, Dong-Mei Yu, Jin-Yong Hu. Accession-specific flowering time variation in response to nitrate fluctuation in Arabidopsis thaliana[J]. Plant Diversity, 2021, 43(01): 78-85.

Figures/Tables 6

References 19

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编号 No.	行政区 District	小区名称 Name	科数 Family	属数 Genus	种数 Species	Shannon-Wiener指数 H′
1	巴南区 Banan District	帝豪巴南印象 Dihaobananyinxiang	6	8	11	1.04
2	巴南区 Banan District	恒大城 Hengdacheng	11	16	21	1.32
3	巴南区 Banan District	融汇半岛 Ronghuibandao	7	12	15	1.18
4	北碚区 Beibei District	华立北泉花园 Hualibeiquanhuayuan	10	16	25	1.40
5	大渡口区 Dadukou District	锦天康都 Jintiankangdu	7	10	13	1.11
6	江北区 Jiangbei District	华宇北国风光 Huayubeiguofengguang	5	7	10	1.00
7	江北区 Jiangbei District	鹏润蓝海 Pengrunlanhai	8	10	15	1.18
8	九龙坡区 Jiulongpo District	金科西城大院 Jinkexichengdayuan	9	9	15	1.18
9	九龙坡区 Jiulongpo District	金科云湖天都 Jinkeyunhutiandu	11	12	19	1.28
10	九龙坡区 Jiulongpo District	协信天骄城 Xiexintianjiaocheng	9	10	15	1.18
11	南岸区 Nan’an District	康德国会山 Kangdeguohuishan	8	10	12	1.08
12	南岸区 Nan’an District	东海长洲 Donghaichangzhou	8	11	16	1.20
13	南岸区 Nan’an District	阳光100国际新城 Yangguang 100 guojixincheng	5	7	8	0.90
14	南岸区 Nan’an District	渝能国际 Yunengguoji	12	14	16	1.20
15	沙坪坝区 Shapingba District	华宇金沙时代 Huayujinshashidai	5	5	7	0.85
16	沙坪坝区 Shapingba District	华宇林泉雅舍 Huayulinquanyashe	5	6	7	0.85
17	沙坪坝区 Shapingba District	蓝溪谷地 Lanxigudi	8	8	10	1.00
18	渝北区 Yubei District	保利香槟花园 Baolixiangbinhuayuan	9	10	14	1.15
19	渝北区 Yubei District	龙湖水晶郦城 Longhushuijinglicheng	6	6	11	1.04
20	渝北区 Yubei District	上品十六 Shangpinshiliu	10	15	23	1.36
21	渝中区 Yuzhong District	嘉华鑫城 Jiahuaxincheng	7	6	10	1.00
22	渝中区 Yuzhong District	兰波红城丽景 Lanbohongchenglijing	3	4	5	0.70
23	渝中区 Yuzhong District	丽水菁苑 Lishuijingyuan	8	13	16	1.20
	总计 Total		19	33	84

编号 No.	行政区 District	小区名称 Name	科数 Family	属数 Genus	种数 Species	Shannon-Wiener指数 H′
1	巴南区 Banan District	帝豪巴南印象 Dihaobananyinxiang	6	8	11	1.04
2	巴南区 Banan District	恒大城 Hengdacheng	11	16	21	1.32
3	巴南区 Banan District	融汇半岛 Ronghuibandao	7	12	15	1.18
4	北碚区 Beibei District	华立北泉花园 Hualibeiquanhuayuan	10	16	25	1.40
5	大渡口区 Dadukou District	锦天康都 Jintiankangdu	7	10	13	1.11
6	江北区 Jiangbei District	华宇北国风光 Huayubeiguofengguang	5	7	10	1.00
7	江北区 Jiangbei District	鹏润蓝海 Pengrunlanhai	8	10	15	1.18
8	九龙坡区 Jiulongpo District	金科西城大院 Jinkexichengdayuan	9	9	15	1.18
9	九龙坡区 Jiulongpo District	金科云湖天都 Jinkeyunhutiandu	11	12	19	1.28
10	九龙坡区 Jiulongpo District	协信天骄城 Xiexintianjiaocheng	9	10	15	1.18
11	南岸区 Nan’an District	康德国会山 Kangdeguohuishan	8	10	12	1.08
12	南岸区 Nan’an District	东海长洲 Donghaichangzhou	8	11	16	1.20
13	南岸区 Nan’an District	阳光100国际新城 Yangguang 100 guojixincheng	5	7	8	0.90
14	南岸区 Nan’an District	渝能国际 Yunengguoji	12	14	16	1.20
15	沙坪坝区 Shapingba District	华宇金沙时代 Huayujinshashidai	5	5	7	0.85
16	沙坪坝区 Shapingba District	华宇林泉雅舍 Huayulinquanyashe	5	6	7	0.85
17	沙坪坝区 Shapingba District	蓝溪谷地 Lanxigudi	8	8	10	1.00
18	渝北区 Yubei District	保利香槟花园 Baolixiangbinhuayuan	9	10	14	1.15
19	渝北区 Yubei District	龙湖水晶郦城 Longhushuijinglicheng	6	6	11	1.04
20	渝北区 Yubei District	上品十六 Shangpinshiliu	10	15	23	1.36
21	渝中区 Yuzhong District	嘉华鑫城 Jiahuaxincheng	7	6	10	1.00
22	渝中区 Yuzhong District	兰波红城丽景 Lanbohongchenglijing	3	4	5	0.70
23	渝中区 Yuzhong District	丽水菁苑 Lishuijingyuan	8	13	16	1.20
	总计 Total		19	33	84

编号 No.	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22
2	0.19
3	0.38	0.44
4	0.22	0.39	0.35
5	0.41	0.41	0.36	0.47
6	0.38	0.25	0.24	0.40	0.43
7	0.08	0.33	0.20	0.35	0.29	0.32
8	0.15	0.33	0.27	0.20	0.21	0	0.33
9	0.13	0.35	0.29	0.32	0.25	0.28	0.24	0.41
10	0.23	0.33	0.33	0.25	0.21	0.16	0.33	0.27	0.29
11	0.09	0.42	0.37	0.32	0.32	0.27	0.37	0.22	0.19	0.37
12	0.22	0.49	0.39	0.34	0.28	0.15	0.32	0.32	0.34	0.39	0.43
13	0.32	0.28	0.35	0.18	0.19	0.22	0.17	0.26	0.22	0.26	0.20	0.33
14	0.30	0.38	0.39	0.34	0.34	0.38	0.39	0.39	0.40	0.32	0.64	0.38	0.17
15	0.33	0.21	0.27	0.13	0.10	0.24	0.18	0.45	0.23	0.27	0.32	0.17	0.27	0.35
16	0.22	0.29	0.18	0.25	0.30	0.12	0.27	0.18	0.31	0.27	0.32	0.26	0.27	0.43	0.29
17	0.19	0.32	0.32	0.23	0.26	0.30	0.24	0.16	0.28	0.40	0.36	0.31	0.22	0.38	0.35	0.47
18	0.24	0.46	0.55	0.46	0.44	0.33	0.41	0.34	0.36	0.28	0.54	0.47	0.36	0.60	0.19	0.38	0.50
19	0.36	0.38	0.38	0.33	0.50	0.57	0.31	0.08	0.20	0.23	0.52	0.30	0.21	0.52	0.33	0.22	0.38	0.48
20	0.24	0.41	0.32	0.29	0.11	0.18	0.37	0.21	0.24	0.26	0.17	0.31	0.06	0.31	0.20	0.20	0.30	0.27	0.24
21	0.29	0.19	0.24	0.34	0.43	0.60	0.24	0.08	0.34	0.24	0.36	0.15	0.22	0.38	0.24	0.24	0.40	0.42	0.48	0.18
22	0.25	0.08	0.20	0.20	0.11	0.27	0.10	0.10	0.25	0.30	0.12	0.29	0	0.19	0.33	0.17	0.27	0.11	0.25	0.14	0.27
23	0.37	0.21	0.26	0.34	0.28	0.38	0.26	0.06	0.17	0.13	0.29	0.31	0.08	0.44	0.17	0.35	0.31	0.33	0.44	0.31	0.31	0.29

编号 No.	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22
2	0.19
3	0.38	0.44
4	0.22	0.39	0.35
5	0.41	0.41	0.36	0.47
6	0.38	0.25	0.24	0.40	0.43
7	0.08	0.33	0.20	0.35	0.29	0.32
8	0.15	0.33	0.27	0.20	0.21	0	0.33
9	0.13	0.35	0.29	0.32	0.25	0.28	0.24	0.41
10	0.23	0.33	0.33	0.25	0.21	0.16	0.33	0.27	0.29
11	0.09	0.42	0.37	0.32	0.32	0.27	0.37	0.22	0.19	0.37
12	0.22	0.49	0.39	0.34	0.28	0.15	0.32	0.32	0.34	0.39	0.43
13	0.32	0.28	0.35	0.18	0.19	0.22	0.17	0.26	0.22	0.26	0.20	0.33
14	0.30	0.38	0.39	0.34	0.34	0.38	0.39	0.39	0.40	0.32	0.64	0.38	0.17
15	0.33	0.21	0.27	0.13	0.10	0.24	0.18	0.45	0.23	0.27	0.32	0.17	0.27	0.35
16	0.22	0.29	0.18	0.25	0.30	0.12	0.27	0.18	0.31	0.27	0.32	0.26	0.27	0.43	0.29
17	0.19	0.32	0.32	0.23	0.26	0.30	0.24	0.16	0.28	0.40	0.36	0.31	0.22	0.38	0.35	0.47
18	0.24	0.46	0.55	0.46	0.44	0.33	0.41	0.34	0.36	0.28	0.54	0.47	0.36	0.60	0.19	0.38	0.50
19	0.36	0.38	0.38	0.33	0.50	0.57	0.31	0.08	0.20	0.23	0.52	0.30	0.21	0.52	0.33	0.22	0.38	0.48
20	0.24	0.41	0.32	0.29	0.11	0.18	0.37	0.21	0.24	0.26	0.17	0.31	0.06	0.31	0.20	0.20	0.30	0.27	0.24
21	0.29	0.19	0.24	0.34	0.43	0.60	0.24	0.08	0.34	0.24	0.36	0.15	0.22	0.38	0.24	0.24	0.40	0.42	0.48	0.18
22	0.25	0.08	0.20	0.20	0.11	0.27	0.10	0.10	0.25	0.30	0.12	0.29	0	0.19	0.33	0.17	0.27	0.11	0.25	0.14	0.27
23	0.37	0.21	0.26	0.34	0.28	0.38	0.26	0.06	0.17	0.13	0.29	0.31	0.08	0.44	0.17	0.35	0.31	0.33	0.44	0.31	0.31	0.29

编号 No.	丛集型 Turfs	交织型 Wefts	平铺型 Mats	树状型 Dendroids	一年生型 Annuals
1	45.45	45.45	9.09	0.00	0.00
2	71.43	19.05	9.52	0.00	0.00
3	66.67	26.67	6.67	0.00	0.00
4	40.00	40.00	12.00	8.00	0.00
5	53.85	38.46	7.69	0.00	0.00
6	20.00	80.00	0.00	0.00	0.00
7	33.33	53.33	13.33	0.00	0.00
8	73.33	6.67	20.00	0.00	0.00
9	52.63	31.58	15.79	0.00	0.00
10	40.00	26.67	33.33	0.00	0.00
11	66.67	25.00	8.33	0.00	0.00
12	62.50	37.50	0.00	0.00	0.00
13	87.50	12.50	0.00	0.00	0.00
14	43.75	43.75	12.50	0.00	0.00
15	57.14	14.29	28.57	0.00	0.00
16	42.86	42.86	14.29	0.00	0.00
17	40.00	50.00	10.00	0.00	0.00
18	42.86	50.00	7.14	0.00	0.00
19	45.45	45.45	9.09	0.00	0.00
20	47.83	39.13	13.04	0.00	0.00
21	20.00	60.00	10.00	0.00	10.00
22	0.00	80.00	20.00	0.00	0.00
23	37.50	56.25	6.25	0.00	0.00