Plant Diversity ›› 2007, Vol. 29 ›› Issue (06): 671-676.

• Articles • Previous Articles     Next Articles

Profiling the Changes of Lipid Molecular Species in Response to
Wounding in Arabidopsis thaliana (Cruciferae) by ESI-MS MS

LING Li-Zhen1 , 2 , 3 , LI Wei-Qi3 , 4, XU Zheng-Jun1 , 2   

  1. 1 Rice Research Institute, Sichuan Agricultural University , Wenjiang 611130 , China; 2 Key Laboratory of Southwest Crop Gene
    Resources and Improvement , Ministry of Education , Sichuan agricultural University, Yaan 625014 , China ;
    3 Kunming Institute of Botany , Chinese Academy of Sciences, Kunming 650204 , China ;
    4 Department of Biology , Honghe University, Mengzi 661100 , China
  • Received:2007-03-05 Online:2007-12-25 Published:2007-12-25
  • Contact: LI Wei-Qi,XU Zheng-Jun

Abstract: This paper employed a sensitive approach based on electrospray ionization tandem mass spectrometry (ESI-MS􊄯MS) to comprehensively analyze lipid composition changes in response tomechanical wounding in Arabidopsis thaliana.The changes of 6 phospholipid classes, 2 glycolipid classes, and 3 lysophospholipid, and 120 lipid molecular species in total depicted the basic
tendency of membrane lipids in response to wounding for 0 , 15, 30 , and 60min in Arabidopsis thaliana . The results showed that wounding can lead to increase phosphatidic acid (PA) and 3 lysophospholipid and decrease glycolipids of the major chloroplast plastidic lipids . The increasing speed and intensity of PA produced by various lipids are very different , which shows those lipids have experienced different biochemistry processes . (1) The accumulation of 34∶4 PA derived from plastidic lipid 34∶4 PG (phosphatidylglycerol) is significantly slower than that of other PA species; (2) 34∶6 PA accumulated a little and possibly derived from the 34∶6 MGDG or DGDG . However , the content of both glycolipids has apparently decreased, which indicated they are possibly involved in other reactions. No remarkable changes of lipid mole percentage were observed .

Key words: Mechanical wounding

CLC Number: