Abstract: A pair of general primer ( ITS5-ITS4) was used to test in amplification of internal transcribed spacer ( ITS) region of chromosomal DNA of fruiting bodies of Boletus edulis in Yunnan. The DNA amplification fragment was purified with DNA Gel Extraction Kit and sequenced by CEQ8000. The result of phylogenetic analyses based on ITS1-5. 8SrDNA-ITS2 sequences showed that B. edulis in Yunnan had high homology with B. aestivalis and B. aereus in Europe. 73 bp and 26 bp DNA characteristic fragments differing from all B. edulis were found in the ITS-region of B. aereus and B. aestivalis respectively.

Key words: Boletus edulis