Abstract: Haploid plantlet and callus were induced from the anther culture of Dioscorea zingiberensis as the explant at the stag of later uninucleate period. The results indicates that the basal culture media and explants from different population affect dramatically callus induction from anthers . W14 was the best basal media for the first step of callus induction. MS media with BA2.0 mg/L + IAA0.2 mg/L improved callus′multiplication and differentiation. MS media with 2.0 mg/L IBA, 0.4 mg/L NAA and 0.5 g/L active carbon were suitable for rooting. Examination of the anther cultured plantlet displayed that 8%- 12% clones were haploid. The established technique system and obtained haploid plant would provide solid support and valuable materials for further breeding of Dioscorea zingiberensis .

Key words: Dioscorea zingiberensis