Plant Diversity ›› 2004, Vol. 26 ›› Issue (01): 1-3.

• Articles •    

Analysis of Genetic Diversity of Trigonobalanus doichangensis (Fagaceae) by RAPD

HAN ChunYan,SUN WeiBang,GAO LianMing   

  1. Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China;Graduate School of Chinese Academy of Sciences,Beijing 100039,China
  • Online:2004-02-25 Published:2004-02-25

Abstract: The genetic diversity and population genetic structure of five populations with total of 99 Trigonobalanus doichangensis individuals were estimated with random amplified polymorphic DNA (RAPD) markers.One hundred and fiftyseven loci were amplified with 16 oligonucleotide primers,of which 83 loci (5287%) were polymorphic.Shannon's information index (I) and Nei's gene diversity (h) were 02431 and 01595 respectively at the species level.Total genetic diversity (Ht) was 01600,and the genetic diversity within populations (Hs) was 00749.The genetic variation between different populations accounted for 5320% of the total.This indicated that genetic variation existed mainly between populations rather than within populations.In comparison with other species from Fagaceae,the genetic diversity of Tdoichangensis is lower and the gene flow among populations is very restricted.These may be attributed to the changes of environment during the species evolution,habitat destruction and excessively making use of the species resources by the people.Based on the research results,the conservation strategies of genetic diversity for the species were put forward.

Key words: Trigonobalanus doichangensis;RAPD;Genetic diversity;Population genetic structure;Conservation