Abstract: CryIAb gene,one of Bt genes,was transferred into the calli of Yunnan riceDianxi No.4 and Hexi No.39 through agrobacterium associated method.Some hygromycin resistant (Hyg+) transformants are regenerated after selection culture with hygromycin.some plantlets of Dianxi No4 and Hexi No.39 were positive in PCR analysis,and some leaves of the plantlets show blue spots in GUS histochemical assay, which indicated that Bt gene was integrated into rice genome.An efficient transformation system was established by this study.In the experiment,we found that the ventilation of the container had great influence on the formation and growth of the calli.Osmotic potential of washing solution and media also affected transformation frequency,because they could reduce the death rate of the calli after washing treatmeat.

Key words: Bt gene；Rice calli；Agrobacterium；Gene transformation；Polymerase chain reaction analysis