Plant Diversity ›› 2003, Vol. 25 ›› Issue (05): 1-3.
• Articles •
JIANG Xiao-Ling YU Shou-Yi HE Zhu-Mei PENG Zhi-Qiang QI Yu
Aim:To optimize the factors that affect transformation system of tomato cotyledon explant.Method:By orthogonal design of statistics,factors that affect transformation such as the Agrobacterium concentration,infiltration time and transform style were assembled.Result:Best transformation system could be set up by the contidition of 2 days preculture,30-fold-dilution,10-minute-infiltation of Agrobacterium solution,and 2 days co-cultivation.The frenquency of success transformation reached to 25%,higher than average level at present.Conclusion:Factors that affect transformation system of tomato cotyledon explant were successfully optimized.
Tomato；Cotyledon explant；Transformation system
JIANG Xiao-Ling YU Shou-Yi HE Zhu-Mei PENG Zhi-Qiang QI Yu. To Optimize the Factors that Affected Transformation System of Tomato Cotyledon Explant[J]. Plant Diversity, 2003, 25(05): 1-3.
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