Abstract: Eighteen compounds,including a new chalcone glycoside (2),were isolated from the aerial parts of Bidens pilosa L.Their structures were elucidated as butein (1)，α,3,2′,4'-tetrahydroxy-2-O-β-D-glucopyranosylchalcone(2),okanin4-methyl ether-3'-O-β-glucoside(3),sulfuretin(4),6,7,3',4'-tetrahydroxyaurone (5),maritimein(6),(Z)-6-O-(6″-acetyl-β-D-glucopyranosyl)6,7,3',4'-tetrahydroxy-aurone(7),(Z)-6-O-(4'',6''-diacetyl-β-D-glucopyranosyl)6,7,3',4'-tetrahydroxy-aurone(8),(Z)-6-O-(3'',4'',6''-triacetyl-β-D-glucopyranosyl)6,7,3',4'-tetrahydroxy-aurone(9),luteolin (10),quercetin (11),isoquercitrin (12),rutin (13),astragalin (14),quercetin3,4'-dimethyl ether-7-O-rutinoside(15),E-butenedioic acid (16)，2-β-D-glucopyranosyloxy-1-hydroxy-trideca-3,5,7,9,11-pentayne (17),3-β-D-glucopyran-osyloxy-1-hydroxy-6(E)-tetrade-cene-8,10,12-triyne(18),respectively,based on the spectral analysis.

Key words: Bidens pilosa