Abstract: The gliadin genetic polymorphism among 45 accessions of 20 species and 1 subspecies in Leymus Hochst.were analyzed by using acid polyacrylamide gel electrophoresis (A-PAGE).The results showed as follows:(1) 43 types of gliadin patterns were obtained from the tested materials and 41 materials had their unique patterns respectively.A total of 38 gliadin bands were separated by electrophoresis,of all showing polymorphism,which amounts to 100%.(2) Distinct gliadin genetic variations were presented among the populations.The gliadin genetic variations among different species were more abundant than that of different accessions,and it revealed that the gliadin patterns can be used as a fingerprint to survey different materials in Leymus Hochst.(3) The relationships among species in Leymus Hochst.according to gliadin data are basically comparable with that obtained from morphological data,so that the gliadin patterns data can be used to investigate genetic variation and biosystematic relationships among species in Leymus Hochst.

Key words: Leymus