Plant Diversity ›› 1987, Vol. 9 ›› Issue (04): 1-3.

• Articles •    

WAYS OF ENHANCING THE ROOTING RATE OF THE IN VITRO CULTURE SHOOTS FROM ADULT CRATAEGUS SCABRIFOLIA TREES

Huang Shizhou, Liu Aiqin, Hu Hong, Duan Jinyu   

  1. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204
  • Online:1987-08-25 Published:1987-08-25

Abstract: The sterile multiplied shoots of the adult Crataegus scabrifolia (Franch) Rehd. were used as the experiment material of rooting. The results sho- wed. shoots inoculated to culture mcdium of MS/ 2 added with auxin (IBA, NAA) at 0.01-1 .0mg/l, the rooting percentage was 50.4%. When the bases of the shoots first were immersed in high auxin concertration (l50--250mg/l) solution for 30 minutes, then inoculated to the culture medium of MS/ 2 devoid of auxin the rooting percentage was 60.1%; if dipping the bases of shoots for 1-2 seconds, the rooting percentage was 83.5%3 if the shoots were cultured for 2- 4 days in MS /2 medium with IBA at 1-5mg/l, then were transfered to the same medium but devoid of auxin, 15 days or so, the rooting percentage was over 92% and roots elongated normal; the rooting almost was not influenced with temperture between 20-29℃; rooting was obviously inhibited in the dark, unsatisfied under light condition 8 hours a day, but very satisfied in 16 hours or 24 hours a day.

Key words: Crataegus scabrifolia