Abstract: Identification by thin-layer chromatography, ultraviolet and visible absorption spectrum of alcohol extracts of callus and stem, leaves of B. pruinosa Franch. proved that the callus had the capability to synthesize berberine. The contents of berberine in the callus was 1.8148, it is slightly higher than the contents of berberine in the stems ofinfact plants (it was 1.58). The growth curves of the callus are sigmoid. When the callus had cultured for about 27 days, the growth rate and contents of berberine were highe than other times.

Key words: Berberis pruinosa