Plant Diversity ›› 1996, Vol. 18 ›› Issue (04): 1-3.
• Articles •
WANG Dong, JI Ben-Ren, ZENG Ying, YU Bing
A trypsin inhibitor named AMTI has been isolated and purified from the rhizome of Alocasia macrorrhiza by a procedure including affinity chromatography on trypsina- Sepharose 4B and gel filtration with Sephadex G-100. This inhibitor was homogeneous on PAGE, SDS-PAGE, IEF and Western blot. It appeared as a single band of about 22 000 dalton on SDS-PAGE. The isoelectric fo- cusing(IEF) showed that its PI was about 6.2. lts inhibitory activity to trypsin indicated that AMTI was a single headed inhibitor. This inhibitor was stable against heat and pH.
WANG Dong, JI Ben-Ren, ZENG Ying, YU Bing. ISOLATION AND CHARACTERIZATION OF A TRYPSIN INHIBITOR FROM ALOCASIA MACRORRHIZA[J]. Plant Diversity, 1996, 18(04): 1-3.
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