Abstract: By using a suitable sample preparation, free and bound phytoestrogens (daidzein, equol, genistein, formononetin, coumestrol and biochanin A) can be isolated from extracts of fresh plant material and subsequently quantified by HPLC at different wave lengths. Chromatographic peaks assigned to phytoestrogens were identified by retention time and by comparison to the addi-tion of standards.

Key words: Phytoestrogens