Plant Diversity ›› 1996, Vol. 18 ›› Issue (01): 1-3.

• Articles •    

MASS PREPARATION OF PLANT CALMODULIN FROM MAIZE GERM

GONG Ming, DU Chao-Kun, YANG Zhong-Han, CAO Zong-Xun (T.H. Tsao)   

  1. Deportment of Life Sciences, Yunnan Normal University, Kunming 650092;Life Science College, Pektw University, Beijing l0087l
  • Online:1996-02-25 Published:1996-02-25

Abstract: 63 mg calmodulin was extracted and purified from l kg acetone powder of malze germ germinated for 24 h, which is the highest record for the quantity of calmoulin purified from l kg plant materials. Our results showed that the calmodulin from maize germ had higher biological ac- tivity, could activate cAMP phosphodiesterase within a good range, and its UV absorption spec-trum, electrophoretic mobility on SDS PAGE in the presence or absence of Ca2+ and amino acid composition was similar to those calmodulins from other plant materials. The above-mentioned results indicated that maize germ is a suitable material for the extraction and purification of plant calmodulin.

Key words: Calmodulin