Plant Diversity ›› 1993, Vol. 15 ›› Issue (01): 1-3.
• Articles •
ZHANG Jiag-Ming ZHENG Yu-Ping CHEN Mei-Ying
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Abstract: Protoplasts isolated from monokaryon mycelia of wild Lentinus edodes in Yunnan and cultivar of Lentinus edodes were fused by applying electric field of sine wave 500kHz, 400pv / cm and square pulse 50us, 500Qpy / cm. Fused protoplasts were cultured into colonies for 10-15 days. The colonies from fusants were selected by discovering clamp connection of hyph^e under the light microscope.
Key words: Lentinus edodes
ZHANG Jiag-Ming ZHENG Yu-Ping CHEN Mei-Ying. PROTOPLAST ELECTROFUSION OF YUNNAN WILD AND CULTIVATED LENTINUS EDODES[J]. Plant Diversity, 1993, 15(01): 1-3.
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https://journal.kib.ac.cn/EN/Y1993/V15/I01/1