Plant Diversity ›› 1993, Vol. 15 ›› Issue (01): 1-3.

• Articles •    

PROTOPLAST ELECTROFUSION OF YUNNAN WILD AND CULTIVATED LENTINUS EDODES

ZHANG Jiag-Ming ZHENG Yu-Ping CHEN Mei-Ying   

  1. Kunming Institute of Botany, Academia Sinica
  • Online:1993-02-25 Published:1993-02-25

Abstract: Protoplasts isolated from monokaryon mycelia of wild Lentinus edodes in Yunnan and cultivar of Lentinus edodes were fused by applying electric field of sine wave 500kHz, 400pv / cm and square pulse 50us, 500Qpy / cm. Fused protoplasts were cultured into colonies for 10-15 days. The colonies from fusants were selected by discovering clamp connection of hyph^e under the light microscope.

Key words: Lentinus edodes