Abstract: Two proteins named ZP-1 and ZP-2 in rhizome of Panaxjaponicus var. major were isolated by the procedure including saline solution extraction, dialysis, ion exchange chromatography, and gel filtration. On PSA media, ZP-2 inhibited obviously the growth of mycelia of Trichodema viride and Fusarium graminearum. The protein contained two subunits (MW. 27 and 28kD), and its molecular weights were 55 and 66kD respectively determined by SDS-PAGE and Sephadex G-75 filtration. By PAS reaction, it was shown that ZP-2 was a glycoprotein, which contained glucose, mannose, fucose, xylose, galactose, rhamnose, and uronic acids. The contents of neutral sugars and uronic acids were 28.91 and 22.76 respectively. The protein was rich in Asp and Glu, but had no activity of hemagglutination, chitinase, and β-1, 3-glucanase.

Key words: Panaxjaponicus var. major