Abstract: Abstract Protoplasts of Morchella conica Pers were isolated from the mycelia cultured in liquid medium. The highest isolated quantity v/as 6.2×10^6 protoplasts/ 100mg·ml in mixed enzyme solution No. 2 and at 30°C tor 4.5 h in cubation. Isolated protoplasts regenerated to hyphae readily. After 18 hours of incubation hyphae grew out from the cells regenerated from the protoplasts. Following plating on solid medium regenerated isolates were obtained.

Key words: Morchella conica