Abstract: The calluses and adventitious buds can be induced from the leaf explant of Dendranthema morifolium (Ramat.)Tzvel. by adding 0.05-0.3 mg/l β-ec- dysone together with 0.5-3.0 mg/l BA(1: 10) to the medium, but roots can not be induced.The formation of roots can be gained by putting β-ecdysone plus NAA in the medium, but buds can not be achieved. The growth of dormant axillary buds can be promoted with 0.5-4.0 mg/l β-ecdysone plus BA or NAA and the roots can be induced from the basal portions of the buds(or shoot) with 0.25-4.0 mg/l β-ecdysone in the medium. So, the effect of β-ecdysone is mainly similar to that of auxins.

Key words: effect, ecdysone, differentiation, explant