Plant Diversity ›› 1982, Vol. 4 ›› Issue (01): 1-3.

• Articles •    

ISOLATION OF AN ONCOGENIC AGROBACTERIUM STRAIN K-8 AND PRELIMINARY DETERMINATION OF SOME ONCOGENIC AGROBACTERIUM STRAINS

Li Ying and Wang Jun   

  1. Kunmming Institute of Botany,Academia sinica
  • Online:1982-02-25 Published:1982-02-25

Abstract: Some oncogenic Agrobacterium strains have positive reaction of 3-ketola-ctate on DYL medium and good growth ability in YBS medium with sucrose as carbon source. Other oncogenic Agrobacterium strains have negatiye reac- tion of 3-ketolactate reaction and bad growth ability in YBS medium. Using this relation, an oncogenic Agrobacterium strain K-8 was isolated from fresh and young crown gall of peach tree with media DYL and YBS. Four oncoge- nic strains isolated from China had been determined. The strain S-1 and strain B-1 are strains of Agrobacterium tumefaciens Biotype I. The strain K-8 and strain 702 are strains of Agrobacterium tumefaciens Biotype II.

Key words: isolation, oncogenic, agrobacterium, preliminary